Early stationary phase culture supernatant accelerates growth of sputum cultures collected after initiation of anti-tuberculosis treatment

We investigated the effect of Mycobacterium tuberculosis culture supernatant added to sputum cultures collected during the first 8 weeks of anti-tuberculosis treatment. With ongoing treatment duration, time to culture positivity decreased significantly in supernatant-enriched cultures, possibly due...

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Published inClinical microbiology and infection Vol. 20; no. 7; pp. O418 - O420
Main Authors Kolwijck, E., Friedrich, S.O., Karinja, M.N., van Ingen, J., Warren, R.M., Diacon, A.H.
Format Journal Article
LanguageEnglish
Published England Elsevier Ltd 01.07.2014
Elsevier Limited
Subjects
Antitubercular Agents - therapeutic use
Culture Media - chemistry
Dormancy
Growth Substances - metabolism
Humans
Mycobacterium tuberculosis
Mycobacterium tuberculosis - growth & development
Mycobacterium tuberculosis - isolation & purification
Mycobacterium tuberculosis - metabolism
resuscitation promoting factors
sputum
Sputum - microbiology
treatment
Tuberculosis, Pulmonary - drug therapy
Tuberculosis, Pulmonary - microbiology
Dormancy
treatment
sputum
Mycobacterium tuberculosis
resuscitation promoting factors
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Summary:We investigated the effect of Mycobacterium tuberculosis culture supernatant added to sputum cultures collected during the first 8 weeks of anti-tuberculosis treatment. With ongoing treatment duration, time to culture positivity decreased significantly in supernatant-enriched cultures, possibly due to stimulation of dormant or slowly metabolizing M. tuberculosis cells.
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ISSN:1198-743X
1469-0691
DOI:10.1111/1469-0691.12441