Cytokinesis in yeast meiosis depends on the regulated removal of Ssp1p from the prospore membrane
Intracellular budding is a developmentally regulated type of cell division common to many fungi and protists. In Saccaromyces cerevisiae, intracellular budding requires the de novo assembly of membranes, the prospore membranes (PSMs) and occurs during spore formation in meiosis. Ssp1p is a sporulati...
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Published in | The EMBO journal Vol. 26; no. 7; pp. 1843 - 1852 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Chichester, UK
John Wiley & Sons, Ltd
04.04.2007
Blackwell Publishing Ltd EMBO Press Nature Publishing Group |
Subjects | |
Online Access | Get full text |
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Summary: | Intracellular budding is a developmentally regulated type of cell division common to many fungi and protists. In Saccaromyces cerevisiae, intracellular budding requires the de novo assembly of membranes, the prospore membranes (PSMs) and occurs during spore formation in meiosis. Ssp1p is a sporulation‐specific protein that has previously been shown to localize to secretory vesicles and to recruit the leading edge protein coat (LEP coat) proteins to the opening of the PSM. Here, we show that Ssp1p is a multidomain protein with distinct domains important for PI(4,5)P2 binding, binding to secretory vesicles and inhibition of vesicle fusion, interaction with LEP coat components and that it is subject to sumoylation and degradation. We found non‐essential roles for Ssp1p on the level of vesicle transport and an essential function of Ssp1p to regulate the opening of the PSM. Together, our results indicate that Ssp1p has a domain architecture that resembles to some extent the septin class of proteins, and that the regulated removal of Ssp1p from the PSM is the major step underlying cytokinesis in yeast sporulation. |
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Bibliography: | istex:E7B1559CC7CB90750EB95B00F1990268E57D7DFA Supplementary Table S1Supplemental Figure LegendsSupplemental Online Materials and Methods and ReferencesSupplementary Movies S1Supplementary Movies S2Supplementary Figure S1Supplementary Figure S2Supplementary Figure S3 ArticleID:EMBJ7601621 ark:/67375/WNG-668646CW-3 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 PMCID: PMC1847655 Present address: Institut Jacques Monod-CNRS, Universites Paris VI and VII, 2 Place Jussieu, 75251 PARIS Cedex 05, France Present address: International Agency for Research on Cancer (IARC), Unit of Gene-Environment Interaction, 150, cours Albert Thomas, 69008 Lyon, France |
ISSN: | 0261-4189 1460-2075 |
DOI: | 10.1038/sj.emboj.7601621 |