The gene expression sequence of radiated mucosa in an animal mucositis model

• Published in: Cell proliferation Vol. 35; no. s1; pp. 93 - 102
• Main Authors: Sonis, S. T., Scherer, J., Phelan, S., Lucey, C. A., Barron, J. E., O'Donnell, K. E., Brennan, R. J., Pan, H., Busse, P., Haley, J. D.
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Science, Ltd 01.08.2002
• Subjects: animal model; Animals; Cricetinae; Disease Models, Animal; DNA Polymerase III - genetics; gene expression; Gene Expression - radiation effects; HSP70 Heat-Shock Proteins - genetics; HSP90 Heat-Shock Proteins - genetics; Male; Mesocricetus; Mouth Mucosa - physiopathology; Mucositis; Nitric Oxide Synthase - genetics; Nitric Oxide Synthase Type II; Oligonucleotide Array Sequence Analysis; Original; radiation; Radiation Injuries, Experimental - genetics; Radiation Injuries, Experimental - physiopathology; Stomatitis - genetics; Stomatitis - physiopathology; Tumor Necrosis Factor-alpha - genetics; Tumor Suppressor Protein p53 - genetics
• ISSN: 0960-7722; 1365-2184
• DOI: 10.1046/j.1365-2184.35.s1.10.x

. Oral mucositis is a common, dose‐limiting, acute toxicity of radiation therapy administered for the treatment of cancers of the head and neck. Accumulating data would suggest that the pathogenesis of mucositis is complex and involves the sequential interaction of all cell types of the oral mucosa, as well as a number of cytokines and elements of the oral environment. While a number of studies have reported on gene expression of particular cell types in response to radiation, the overall response of irradiated mucosa has only been evaluated in a limited way. The present study was undertaken to evaluate the expression of a target group of genes using RNA quantification assays and, more broadly, to assess patterns of mucosal gene expression using DNA microarray hybridization. Our results demonstrate the sequential upregulation of a series of genes that, when taken collectively, suggest an intricate functional interaction.