纳米铜/石墨烯修饰电极用于离子交换色谱测定五种单糖
把纳米铜和石墨烯修饰在玻碳电极表面,制备了纳米铜/石墨烯复合修饰电极。采用电化学方法,在-1.5—0V的循环扫描电位条件下,氧化石墨烯(GO)和Cu^2+同时在玻碳电极上被电化学还原,形成石墨烯(Gr)和纳米铜(CuNPs)复合膜。所制备的修饰电极对葡萄糖等单糖化合物具有较高的电催化活性,且电极稳定性和重现性均良好。将此修饰电极作为电化学检测器,与高效阴离子交换色谱联用,分离测定了5种单糖化合物(岩藻糖、阿拉伯糖、半乳糖、葡萄糖和甘露糖)。结果表明,岩藻糖和阿拉伯糖的线性范围为0.1~100mg/L,半乳糖、葡萄糖和甘露糖的线性范围为0.5~100mg/L,5种单糖化合物的线性相关系数均大于0...
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Published in | 分析化学 Vol. 41; no. 10; pp. 1601 - 1605 |
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Main Author | |
Format | Journal Article |
Language | Chinese |
Published |
浙江大学西溪校区化学系,杭州,310028%浙江大学医学院附属妇产科医院,杭州,310006
2013
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Subjects | |
Online Access | Get full text |
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Summary: | 把纳米铜和石墨烯修饰在玻碳电极表面,制备了纳米铜/石墨烯复合修饰电极。采用电化学方法,在-1.5—0V的循环扫描电位条件下,氧化石墨烯(GO)和Cu^2+同时在玻碳电极上被电化学还原,形成石墨烯(Gr)和纳米铜(CuNPs)复合膜。所制备的修饰电极对葡萄糖等单糖化合物具有较高的电催化活性,且电极稳定性和重现性均良好。将此修饰电极作为电化学检测器,与高效阴离子交换色谱联用,分离测定了5种单糖化合物(岩藻糖、阿拉伯糖、半乳糖、葡萄糖和甘露糖)。结果表明,岩藻糖和阿拉伯糖的线性范围为0.1~100mg/L,半乳糖、葡萄糖和甘露糖的线性范围为0.5~100mg/L,5种单糖化合物的线性相关系数均大于0.998,相对标准偏差RSD(n=6)为1.9%~2.5%,检出限在0.02~0.10mg/L之间;将此方法用于测定样品桑黄粗多糖的单糖组成,测得5种单糖的回收率为84.8%-94.5%,准确度和精密度均较好。 |
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Bibliography: | A novel copper nanoparticles/graphene modified glassy carbon electrode (CuNPs-Gr/GCE) was constructed. The CuNPs-Gr nanoeomposite was prepared by simultaneous electrochemical reduction using graphene oxide (GO) and copper ion as the starting materials under cyclic potential from -1.5 to 0 V. The modified electrode showed prominent electrocatalytic oxidation towards monosaccharide. Employing the CuNPs- Gr/GCE as an amperometric detector for high performance anion-exchange chromatography (HPIC), five monosaccharides (fucose, arabinose, galactose, glucose and mannose ) were analyzed on-line with good reproducibility and stablity. Under constant applied potential of +0.55 V (vs Ag/AgC1), the detection limits (S/N= 3 ) of the all investigated compounds were at the level of 0.02-0.10 mg/L. The dynamic linear ranges spanned generally three orders of magnitude with correlation coefficients more than 0. 998, and the RSDs ( n = 6) range from 1. 9% to 2. 5%. The analytical method has successfully been applied to determi |
ISSN: | 0253-3820 |
DOI: | 10.3724/SP.J.1096.2013.30498 |