5个紫菜属物种丝状体的微卫星初步遗传分析
文章利用68对微卫星引物对坛紫菜（Porphyra haitanensis）、半叶紫菜（P.katadai）、条斑紫菜（P.yezoensis）、少精紫菜（P.oligospermatangia）和皱紫菜（P.crispata）5个紫菜属物种的丝状体进行初步的遗传分析,共筛选出20对高多态性适用引物,在10份紫菜丝状体样品扩增位点的多态性比例为95.24%,每对引物可扩增的等位基因位点数为1.22～4.05,平均为2.37;各样品之间的遗传多样性指数为0.254 6～0.899 4;不同物种的遗传距离为0.106 0～1.406 6,同一物种的丝状体材料优先聚类为一支,表明筛选出的微卫星引物具...
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| Published in | 南方水产科学 Vol. 8; no. 4; pp. 29 - 36 |
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| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
汕头大学海洋生物研究所,广东汕头,515063%中国海洋大学海洋生命学院,山东青岛,266003
2012
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| Subjects | |
| Online Access | Get full text |
| ISSN | 2095-0780 |
| DOI | 10.3969/j.issn.2095-0780.2012.04.005 |
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| Abstract | 文章利用68对微卫星引物对坛紫菜（Porphyra haitanensis）、半叶紫菜（P.katadai）、条斑紫菜（P.yezoensis）、少精紫菜（P.oligospermatangia）和皱紫菜（P.crispata）5个紫菜属物种的丝状体进行初步的遗传分析,共筛选出20对高多态性适用引物,在10份紫菜丝状体样品扩增位点的多态性比例为95.24%,每对引物可扩增的等位基因位点数为1.22～4.05,平均为2.37;各样品之间的遗传多样性指数为0.254 6～0.899 4;不同物种的遗传距离为0.106 0～1.406 6,同一物种的丝状体材料优先聚类为一支,表明筛选出的微卫星引物具有良好的遗传分辨能力。 |
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| AbstractList | 文章利用68对微卫星引物对坛紫菜（Porphyra haitanensis）、半叶紫菜（P.katadai）、条斑紫菜（P.yezoensis）、少精紫菜（P.oligospermatangia）和皱紫菜（P.crispata）5个紫菜属物种的丝状体进行初步的遗传分析,共筛选出20对高多态性适用引物,在10份紫菜丝状体样品扩增位点的多态性比例为95.24%,每对引物可扩增的等位基因位点数为1.22～4.05,平均为2.37;各样品之间的遗传多样性指数为0.254 6～0.899 4;不同物种的遗传距离为0.106 0～1.406 6,同一物种的丝状体材料优先聚类为一支,表明筛选出的微卫星引物具有良好的遗传分辨能力。 S917.3; 文章利用68对微卫星引物对坛紫菜(Porphyra haitanensis)、半叶紫菜(P.katadai)、条斑紫菜(P.yezoensis、少精紫菜(P.oligospermatangia)和皱紫菜(P.crispata)5个紫菜属物种的丝状体进行初步的遗传分析,共筛选出20对高多态性适用引物,在10份紫菜丝状体样品扩增位点的多态性比例为95.24％,每对引物可扩增的等位基因位点数为1.22～4.05,平均为2.37;各样品之间的遗传多样性指数为0.2546 ～0.8994;不同物种的遗传距离为0.1060～1.4066,同一物种的丝状体材料优先聚类为一支,表明筛选出的微卫星引物具有良好的遗传分辨能力. |
| Author | 吴文婷 张磊 宋志民 赵翠 汤晓荣 刘涛 陈伟洲 |
| AuthorAffiliation | 汕头大学海洋生物研究所,广东汕头515063 中国海洋大学海洋生命学院,山东青岛266003 |
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| Author_FL | SONG Zhimin LIU Tao WU Wenting ZHANG Lei ZHAO Cui TANG Xiaorong CHEN Weizhou |
| Author_FL_xml | – sequence: 1 fullname: WU Wenting – sequence: 2 fullname: ZHANG Lei – sequence: 3 fullname: SONG Zhimin – sequence: 4 fullname: ZHAO Cui – sequence: 5 fullname: TANG Xiaorong – sequence: 6 fullname: LIU Tao – sequence: 7 fullname: CHEN Weizhou |
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| Copyright | Copyright © Wanfang Data Co. Ltd. All Rights Reserved. |
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| DOI | 10.3969/j.issn.2095-0780.2012.04.005 |
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| DocumentTitleAlternate | Preliminary genetic analysis of conchocelis from 5 Porphyra species using microsateUite |
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| EndPage | 36 |
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| Keywords | 遗传分化 微卫星 等位基因位点 紫菜 |
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| Notes | Porphyra; microsatellites ; allele loci; genetic variation We conducted a preliminary genetic analysis of conchocelis from 5 Porphyra species（P.haitanensis,P.katadai,P.yezoensis,P.oligospermatangia and P.crispate） by 68 microsatellite primer-pairs,and selected 20 microsatellite primer-pairs with high polymorphism.The polymorphism proportion among 10 Porphyra conchocelis reaches 95.24%,and the effective number of alleles for each primer is 1.22～4.05（average 2.37）.The genetic diversity index is 0.254 6～0.899 4 and the genetic distance between different species is 0.106 0～1.406 6.Conchocelis from the same species group into one cluster first,which reveals that the selected microsatellite primer-pairs have good genetic resolution power. WU Wenting1,ZHANG Lei2,SONG Zhimin1,ZHAO Cui2,TANG Xiaorong2, LIU Tao2,CHEN Weizhou1（1.Marine Biology Institute,Shantou University,Shantou 515063,China; 2.College of Marine Life Sciences,Ocean University of China,Qingdao 266003,China） 44-1683/S |
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| PublicationTitle | 南方水产科学 |
| PublicationTitleAlternate | South China Fisheries Science |
| PublicationTitle_FL | South China Fisheries Science |
| PublicationYear | 2012 |
| Publisher | 汕头大学海洋生物研究所,广东汕头,515063%中国海洋大学海洋生命学院,山东青岛,266003 |
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| SubjectTerms | 微卫星 等位基因位点 紫菜 遗传分化 |
| Title | 5个紫菜属物种丝状体的微卫星初步遗传分析 |
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