5个紫菜属物种丝状体的微卫星初步遗传分析

文章利用68对微卫星引物对坛紫菜(Porphyra haitanensis)、半叶紫菜(P.katadai)、条斑紫菜(P.yezoensis)、少精紫菜(P.oligospermatangia)和皱紫菜(P.crispata)5个紫菜属物种的丝状体进行初步的遗传分析,共筛选出20对高多态性适用引物,在10份紫菜丝状体样品扩增位点的多态性比例为95.24%,每对引物可扩增的等位基因位点数为1.22~4.05,平均为2.37;各样品之间的遗传多样性指数为0.254 6~0.899 4;不同物种的遗传距离为0.106 0~1.406 6,同一物种的丝状体材料优先聚类为一支,表明筛选出的微卫星引物具...

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Published in南方水产科学 Vol. 8; no. 4; pp. 29 - 36
Main Author 吴文婷 张磊 宋志民 赵翠 汤晓荣 刘涛 陈伟洲
Format Journal Article
LanguageChinese
Published 汕头大学海洋生物研究所,广东汕头,515063%中国海洋大学海洋生命学院,山东青岛,266003 2012
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ISSN2095-0780
DOI10.3969/j.issn.2095-0780.2012.04.005

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Summary:文章利用68对微卫星引物对坛紫菜(Porphyra haitanensis)、半叶紫菜(P.katadai)、条斑紫菜(P.yezoensis)、少精紫菜(P.oligospermatangia)和皱紫菜(P.crispata)5个紫菜属物种的丝状体进行初步的遗传分析,共筛选出20对高多态性适用引物,在10份紫菜丝状体样品扩增位点的多态性比例为95.24%,每对引物可扩增的等位基因位点数为1.22~4.05,平均为2.37;各样品之间的遗传多样性指数为0.254 6~0.899 4;不同物种的遗传距离为0.106 0~1.406 6,同一物种的丝状体材料优先聚类为一支,表明筛选出的微卫星引物具有良好的遗传分辨能力。
Bibliography:Porphyra; microsatellites ; allele loci; genetic variation
We conducted a preliminary genetic analysis of conchocelis from 5 Porphyra species(P.haitanensis,P.katadai,P.yezoensis,P.oligospermatangia and P.crispate) by 68 microsatellite primer-pairs,and selected 20 microsatellite primer-pairs with high polymorphism.The polymorphism proportion among 10 Porphyra conchocelis reaches 95.24%,and the effective number of alleles for each primer is 1.22~4.05(average 2.37).The genetic diversity index is 0.254 6~0.899 4 and the genetic distance between different species is 0.106 0~1.406 6.Conchocelis from the same species group into one cluster first,which reveals that the selected microsatellite primer-pairs have good genetic resolution power.
WU Wenting1,ZHANG Lei2,SONG Zhimin1,ZHAO Cui2,TANG Xiaorong2, LIU Tao2,CHEN Weizhou1(1.Marine Biology Institute,Shantou University,Shantou 515063,China; 2.College of Marine Life Sciences,Ocean University of China,Qingdao 266003,China)
44-1683/S
ISSN:2095-0780
DOI:10.3969/j.issn.2095-0780.2012.04.005