Autoantibody Profiling and Anti-Kinesin Reactivity in ANCA-Associated Vasculitis

ANCA-associated vasculitides (AAV) are rare autoimmune diseases causing inflammation and damage to small blood vessels. New autoantibody biomarkers are needed to improve the diagnosis and treatment of AAV patients. In this study, we aimed to profile the autoantibody repertoire of AAV patients using...

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Published inInternational journal of molecular sciences Vol. 24; no. 20; p. 15341
Main Authors Mescia, Federica, Bayati, Shaghayegh, Brouwer, Elisabeth, Heeringa, Peter, Toonen, Erik J. M, Beenes, Marijke, Ball, Miriam J, Rees, Andrew J, Kain, Renate, Lyons, Paul A, Nilsson, Peter, Pin, Elisa
Format Journal Article
LanguageEnglish
Published Basel MDPI AG 01.10.2023
MDPI
Subjects
ANCA-associated vasculitis
Antibodies
Antigens
Autoantibodies
autoantibody profiling
Autoimmunity
biomarkers
Disease
Inflammation
Kinesin
kinesins
Medical research
Medicine, Experimental
Neutrophils
protein array
Proteins
Vasculitis
United Kingdom
Sweden
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Summary:ANCA-associated vasculitides (AAV) are rare autoimmune diseases causing inflammation and damage to small blood vessels. New autoantibody biomarkers are needed to improve the diagnosis and treatment of AAV patients. In this study, we aimed to profile the autoantibody repertoire of AAV patients using in-house developed antigen arrays to identify previously unreported antibodies linked to the disease per se, clinical subgroups, or clinical activity. A total of 1743 protein fragments representing 1561 unique proteins were screened in 229 serum samples collected from 137 AAV patients at presentation, remission, and relapse. Additionally, serum samples from healthy individuals and patients with other type of vasculitis and autoimmune-inflammatory conditions were included to evaluate the specificity of the autoantibodies identified in AAV. Autoreactivity against members of the kinesin protein family were identified in AAV patients, healthy volunteers, and disease controls. Anti-KIF4A antibodies were significantly more prevalent in AAV. We also observed possible associations between anti-kinesin antibodies and clinically relevant features within AAV patients. Further verification studies will be needed to confirm these findings.
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ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms242015341