Sonic-assisted antibacterial photodynamic therapy: a strategy for enhancing lateral canal disinfection

• Published in: BMC oral health Vol. 24; no. 1; p. 5
• Main Authors: Wang, Yanhuang, Lei, Lishan, Huang, Jing, Cai, Zhiyu, Huang, Xiaojing
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 02.01.2024; BioMed Central; BMC
• Subjects: Anti-Bacterial Agents - pharmacology; Anti-Bacterial Agents - therapeutic use; Antibacterial activity; Antibacterial agents; Antimicrobial agents; Antimicrobial photodynamic therapy; Bacteria; Biofilms; Cancer; Care and treatment; Complications and side effects; Dental Pulp Cavity - microbiology; Dentin; Disinfection; Disinfection - methods; Dosage and administration; Drug resistance; Endodontics; Enterococcal infections; Enterococcus faecalis; Ethylenediaminetetraacetic acid; Health aspects; Humans; Infection; Lateral canal; Medical research; Medicine, Experimental; Methyl salicylate; Methylene blue; Microorganisms; Patient outcomes; Permeability; Photochemotherapy; Photochemotherapy - methods; Photodynamic therapy; Photosensitizing Agents - pharmacology; Photosensitizing Agents - therapeutic use; Premolars; Root Canal Irrigants - pharmacology; Root Canal Irrigants - therapeutic use; Root canal therapy; Root canals; Sample size; Sodium hypochlorite; Sodium Hypochlorite - pharmacology; Sodium Hypochlorite - therapeutic use; Sonic; Statistical analysis; Teeth; Toxicity; China; Germany; Enterococcus faecalis; Sonic; Lateral canal; Antimicrobial photodynamic therapy; Methylene blue
• ISSN: 1472-6831; 1472-6831
• DOI: 10.1186/s12903-023-03801-6

Bacterial infections in lateral canals pose challenges for root canal treatment. This in vitro study aims to evaluate the antibacterial efficacy of sonic-assisted methylene blue mediated antimicrobial photodynamic therapy (MB-aPDT) against Enterococcus faecalis (E. faecalis) in infected lateral canals. Sixty-five premolars infected with E. faecalis in lateral canals were randomly divided into five groups (n = 13) and treated with : (1) 5.25% NaOCl (positive control); (2) Saline (negative control); (3) Sonic-assisted MB-aPDT; (4) 3% NaOCl + MB-aPDT; (5) 3% NaOCl + sonic-assisted MB-aPDT, respectively. The antibacterial efficacy was evaluated by the colony- counting method (CCM) and scanning electronic microscope (SEM). Both 5.25% NaOCl and the 3% NaOCl + sonic-assisted MB-aPDT exhibited the most effective while comparable antibacterial effects without significant statistical difference (P > 0.05). Furthermore, the antibacterial effect of the 3% NaOCl + MB-aPDT group was significantly higher compared to that of the sonic-assisted MB-aPDT group (P < 0.05). The SEM results demonstrated notable morphological alterations in E. faecalis across all experimental groups, except for the negative control group. The concentration of NaOCl can be reduced to a safe level while preserving its antibacterial efficacy through the synergism with the sonic-assisted MB-aPDT in this study.