A light stabilizer Tinuvin 770-induced toxic injury of adult rat cardiac myocytes

• Published in: Forensic science international Vol. 119; no. 3; pp. 322 - 327
• Main Authors: Sótonyi, P., Keller, É., Járay, J., Nemes, B., Benkõ, T., Kovács, A., Tolokán, A., Rajs, I.
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ireland Ltd 15.07.2001; Elsevier; Elsevier Limited
• Subjects: Adenosine triphosphate; Animals; Biological activity; Biological and medical sciences; bis(2,2,6,6-tetramethyl-4-piperidinyl)sebacate; BTMPS; Calcium channels (L-type); Calcium ions; Cardiomyocytes; Cell Membrane - ultrastructure; Cells, Cultured; Chemical and industrial products toxicology. Toxic occupational diseases; Decanoic Acids - toxicity; Electron microscopy; Enzymes; Fatty acids; Forensic sciences; Heart; Heart - drug effects; Isolated cardiomyocytes; Laboratories; Light; Male; Medical sciences; Medicin och hälsovetenskap; Microbleb; Morphology; Myocytes; Organic solvents; Phenylalkylamine; Physiology; Piperidines - toxicity; Plasma; Polyolefins; Polypropylene; Rats; Rats, Sprague-Dawley; Statistical significance; Tinuvin 770; Toxic cell injury; Toxicology; Tubes; Various organic compounds; United States > US; Isolated cardiomyocytes; Tinuvin 770; Electron microscopy; Toxic cell injury; BTMPS; Microbleb; Rat; Toxicity; Rodentia; Cytotoxicity; Experimental study; In vitro; Biological activity; Myocyte; Sebacate polymer; Vertebrata; Mammalia; Animal; Myocardium; Light stabiliser
• ISSN: 0379-0738; 1872-6283
• DOI: 10.1016/S0379-0738(00)00462-X

Tinuvin 770/bis(2,2,6,6-tetramethyl-4-piperidinyl)sebacate is a worldwide used light stabilizer for plastic materials like polyolefins. Tinuvin 770 is a biologically active component of polypropylene tubes. Glossmann and his study group managed to extract this compound by aqueous or organic solvents from laboratory plastic tubes, and propose that Tinuvin 770 is a potent blocker of L-type Ca 2+-channel through the phenylalkylamine and benzothiazepine-selective drug binding domains of the α 1 subunit of the receptor [Proc. Natl. Acad. Sci. U.S.A. 90 (1993) 9523]. We examined the direct morphological effect of Tinuvin 770 in give 25 nmol, 0, 30, 60, 120 minute exposure time in isolated cardiomyocytes from adult rats. Incubation of myocytes with Tinuvin resulted in a progressive decline of rod-shaped and viable cells. It was accompanied by an increase in number of hypercontracted myocytes with microbleb formation compared to control and depletion of ATP level. In summary, our results demonstrate that plasma membrane damage and hypercontraction are manifestations of Tinuvin-induced injury of isolated cardiomyocytes.