Towards multiplexed immunofluorescence of 3D tissues

Profiling molecular expression in situ allows the integration of biomolecular and cellular features, enabling an in-depth understanding of biological systems. Multiplexed immunofluorescence methods can visualize tens to hundreds of proteins from individual tissue samples, but their application is us...

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Bibliographic Details
Published inMolecular brain Vol. 16; no. 1; p. 37
Main Authors Cho, Wonjin, Kim, Sehun, Park, Young-Gyun
Format Journal Article
LanguageEnglish
Published England BioMed Central Ltd 02.05.2023
BioMed Central
BMC
Subjects
3D immunostaining
Algorithms
Antibodies
Blood vessels
Cellular proteins
Deoxyribonucleic acid
DNA
Fluorescent Antibody Technique
Immunofluorescence
Methods
Microscopy
Multiplexed immunofluorescence
Nano Review
Proteins
Registration
New York
United States > US
Multiplexed immunofluorescence
Immunofluorescence
3D immunostaining
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Summary:Profiling molecular expression in situ allows the integration of biomolecular and cellular features, enabling an in-depth understanding of biological systems. Multiplexed immunofluorescence methods can visualize tens to hundreds of proteins from individual tissue samples, but their application is usually limited to thin tissue sections. Multiplexed immunofluorescence of thick tissues or intact organs will enable high-throughput profiling of cellular protein expression within 3D tissue architectures (e.g., blood vessels, neural projections, tumors), opening a new dimension in diverse biological research and medical applications. We will review current multiplexed immunofluorescence methods and discuss possible approaches and challenges to achieve 3D multiplexed immunofluorescence.
Bibliography:ObjectType-Article-2
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ISSN:1756-6606
1756-6606
DOI:10.1186/s13041-023-01027-9