Redox-mediated substrate recognition by Sdp1 defines a new group of tyrosine phosphatases

• Published in: Nature Vol. 447; no. 7143; pp. 487 - 492
• Main Authors: McDonald, N. Q, Fox, G. C, Shafiq, M, Briggs, D. C, Knowles, P. P, Collister, M, Didmon, M. J, Makrantoni, V, Dickinson, R. J, Hanrahan, S, Totty, N, Stark, M. J. R, Keyse, S. M
• Format: Journal Article
• Language: English
• Published: London Nature Publishing 24.05.2007; Nature Publishing Group
• Subjects: Amino Acid Sequence; Binding Sites; Biochemistry; Biological and medical sciences; Catalysis; Cell physiology; Cellular biology; Cysteine - metabolism; Disulfides - metabolism; Dual-Specificity Phosphatases; Fundamental and applied biological sciences. Psychology; Fungi - enzymology; Histidine - metabolism; Humans; Kinases; Models, Molecular; Molecular and cellular biology; Molecular Sequence Data; Molecular structure; Oxidation; Oxidation-Reduction - drug effects; Oxidative Stress; Phosphoprotein Phosphatases - chemistry; Phosphoprotein Phosphatases - classification; Phosphoprotein Phosphatases - metabolism; Phosphotyrosine - metabolism; Protein Tyrosine Phosphatases - chemistry; Protein Tyrosine Phosphatases - classification; Protein Tyrosine Phosphatases - metabolism; Saccharomyces cerevisiae Proteins - chemistry; Saccharomyces cerevisiae Proteins - classification; Saccharomyces cerevisiae Proteins - metabolism; Signal transduction; Substrate Specificity; Substrates; Taxa; Yeasts; Substrate; Oxidative stress; Enzyme; Phosphoprotein phosphatase; Phosphoric monoester hydrolases; Mitogen-activated protein kinase; Hydrolases; Esterases; Dephosphorylation; Recognition; Cell signaling; Protein-tyrosine-phosphatase
• ISSN: 0028-0836; 1476-4687; 1476-4679
• DOI: 10.1038/nature05804

Reactive oxygen species trigger cellular responses by activation of stress-responsive mitogen-activated protein kinase (MAPK) signalling pathways. Reversal of MAPK activation requires the transcriptional induction of specialized cysteine-based phosphatases that mediate MAPK dephosphorylation. Paradoxically, oxidative stresses generally inactivate cysteine-based phosphatases by thiol modification and thus could lead to sustained or uncontrolled MAPK activation. Here we describe how the stress-inducible MAPK phosphatase, Sdp1, presents an unusual solution to this apparent paradox by acquiring enhanced catalytic activity under oxidative conditions. Structural and biochemical evidence reveals that Sdp1 employs an intramolecular disulphide bridge and an invariant histidine side chain to selectively recognize a tyrosine-phosphorylated MAPK substrate. Optimal activity critically requires the disulphide bridge, and thus, to the best of our knowledge, Sdp1 is the first example of a cysteine-dependent phosphatase that couples oxidative stress with substrate recognition. We show that Sdp1, and its paralogue Msg5, have similar properties and belong to a new group of phosphatases unique to yeast and fungal taxa.