Thermaerobacter composti sp. nov., a novel extremely thermophilic bacterium isolated from compost

• Published in: Journal of general and applied microbiology Vol. 55; no. 5; pp. 323 - 328
• Main Authors: Yabe, Shuhei, Kato, Atsushi, Hazaka, Masaru, Yokota, Akira
• Format: Journal Article
• Language: English
• Published: Japan Applied Microbiology, Molecular and Cellular Biosciences Research Foundation 2009; Japan Science and Technology Agency
• Subjects: Bacteria; Bacteria, Aerobic - classification; Bacteria, Aerobic - genetics; Bacteria, Aerobic - isolation & purification; Base Composition; compost; DNA, Ribosomal - analysis; extremely thermophilic bacterium; Hot Temperature; Japan; Molecular Sequence Data; RNA, Ribosomal, 16S - analysis; RNA, Ribosomal, 16S - isolation & purification; Soil; Thermaerobacter; Thermaerobacter composti sp. nov; Thermaerobacter marianensis; Thermaerobacter nagasakiensis
• ISSN: 0022-1260; 1349-8037
• DOI: 10.2323/jgam.55.323

A novel extremely thermophilic bacterium (Ni80T) was isolated from food sludge compost in Japan. Cells were Gram-positive, spore-forming, strictly-aerobic and rod-shaped with peritrichous flagella. The novel isolate grew at pH 6.5-10.5 but not at pH 11.0 (optimum pH 8.0) and 52-79°C but not 80°C (optimum 70°C). The strain could not grow in more than 1% NaCl. The G+C content of the genomic DNA is 72 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain Ni80T belonged to the genus Thermaerobacter, and that the strain was most closely related to Thermaerobacter subterraneus ATCC BAA-137T (98.2%), Thermaerobacter nagasakiensis JCM 11223T (98.2%), Thermaerobacter marianensis JCM 10246T (98.0%) and Thermaerobacter litoralis JCM 13210T (97.7%). DNA-DNA hybridization showed relatedness values of less than 10% with these species. On the basis of phenotypic and molecular data, strain Ni80T represent a novel species of the genus Thermaerobacter, for which the name Thermaerobacter composti sp. nov. is proposed. The type strain is Ni80T (=JCM 15650T =ATCC BAA-1731T).