Pyrosequencing of the Genital Microbiotas of HIV-Seropositive and -Seronegative Women Reveals Lactobacillus iners as the Predominant Lactobacillus Species

• Published in: Applied and Environmental Microbiology Vol. 77; no. 1; pp. 378 - 381
• Main Authors: Spear, Gregory T, Gilbert, Douglas, Landay, Alan L, Zariffard, Reza, French, Audrey L, Patel, Pranjal, Gillevet, Patrick M
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 01.01.2011; American Society for Microbiology (ASM)
• Subjects: Biodiversity; Biological and medical sciences; Cluster Analysis; DNA, Bacterial - chemistry; DNA, Bacterial - genetics; DNA, Ribosomal - chemistry; DNA, Ribosomal - genetics; Female; Fundamental and applied biological sciences. Psychology; Genes; Gram-positive bacteria; HIV; HIV Infections; Human immunodeficiency virus; Humans; Lactobacillus; Lactobacillus - classification; Lactobacillus - genetics; Lactobacillus - isolation & purification; Metagenome; Microbiology; Pathogens; Phylogeny; Public Health Microbiology; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; Urogenital system; Vagina - microbiology; Women; Virus; Lactic acid bacteria; Retroviridae; Bacteria; Lactobacillaceae; Microflora; Human immunodeficiency virus; Lentivirus; Lactobacillus
• ISSN: 0099-2240; 1098-5336
• DOI: 10.1128/AEM.00973-10

The species of vaginal lactobacilli in HIV-seropositive and -seronegative women were determined by 16S gene pyrosequencing. Lactobacillus iners sequences were the predominant lactobacillus sequences in 66% of HIV⁺ women and 90% of HIV⁻ women. This has implications for resistance of HIV⁺ and HIV⁻ women to genital colonization by pathogenic organisms.