TFEB, a potential therapeutic target for osteoarthritis via autophagy regulation

• Published in: Cell death & disease Vol. 9; no. 9; pp. 858 - 15
• Main Authors: Zheng, Gang, Zhan, Yu, Li, Xiaobin, Pan, Zongyou, Zheng, Fanghong, Zhang, Zengjie, Zhou, Yifei, Wu, Yaosen, Wang, Xiangyang, Gao, Weiyang, Xu, Huazi, Tian, Naifeng, Zhang, Xiaolei
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 28.08.2018; Springer Nature B.V
• Subjects: 13; 13/2; 14/1; 14/35; 14/63; Aged; Animal models; Animals; Antibodies; Apoptosis; Apoptosis - drug effects; Apoptosis - physiology; Arthritis; Autophagy; Autophagy - drug effects; Autophagy - physiology; Basic Helix-Loop-Helix Leucine Zipper Transcription Factors - metabolism; Biochemistry; Butyl hydroperoxide; Cartilage; Cartilage diseases; Cell Biology; Cell Culture; Chloroquine; Chloroquine - pharmacology; Chondrocytes; Chondrocytes - drug effects; Chondrocytes - metabolism; Disease Models, Animal; Female; Gene Expression Regulation - drug effects; Gene Expression Regulation - physiology; Humans; Immunology; Life Sciences; Localization; Lysosomes - drug effects; Lysosomes - metabolism; Male; Meniscus; Mice; Mice, Inbred C57BL; Middle Aged; Osteoarthritis; Osteoarthritis - drug therapy; Osteoarthritis - metabolism; Phagocytosis; Senescence; Signal Transduction - drug effects; Signal Transduction - physiology; Surgery; tert-Butylhydroperoxide - pharmacology; Therapeutic applications; Therapeutic targets
• ISSN: 2041-4889; 2041-4889
• DOI: 10.1038/s41419-018-0909-y

The blockage of autophagic flux in chondrocytes has been considered as a major reason for the excessive cellular apoptosis and senescence in osteoarthritis (OA) development; however, the molecular mechanism and therapeutic strategy for interrupted autophagic flux is still not clear. Most recently, the transcription factor EB (TFEB) is identified as a master regulator for autophagic flux via initiating the expression of multiple autophagy-related genes and lysosomal biogenesis. This research was performed to confirm whether TFEB expression and activity are impacted in OA development and to confirm the effect of genetic up-regulation of TFEB on autophagic flux and cellular protection in the in vitro and in vivo models of OA. We demonstrated that the expression and nuclear localization of TFEB is decreased in human and mouse OA cartilage as well as in tert-Butyl hydroperoxide (TBHP)-treated chondrocytes. Applying lentivirus to transfect chondrocytes, we found that TFEB overexpression rescues the TBHP-induced the autophagic flux damage, lysosome dysfunction and protects chondrocyte against TBHP induced apoptosis and senescence; these protections of TFEB are diminished by chloroquine-medicated autophagy inhibition. Our destabilized medial meniscus (DMM) mouse OA model shows that TFEB overexpression ameliorates the surgery-induced cartilage degradation, restrains the apoptosis and senescence of chondrocyte, and enhances the autophagic flux. In summary, our study indicates that the activity of TFEB in chondrocyte is involved in OA development, also TFEB overexpression may be a promising strategy for OA treatment.