Analgesia by intrathecal delta-9-tetrahydrocannabinol is dependent on Cav3.2 calcium channels

• Published in: Molecular brain Vol. 16; no. 1; p. 47
• Main Authors: Gadotti, Vinicius de Maria, Antunes, Flavia Tasmin Techera, Zamponi, Gerald W
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 25.05.2023; BioMed Central; BMC
• Subjects: Analgesia; Analgesics; Analgesics - pharmacology; Analysis; Animals; Calcium channels; Calcium channels (voltage-gated); Calcium Channels, T-Type; Cannabidiol; Cannabinoid CB1 receptors; Cannabinoid CB2 receptors; Cannabinoid receptors; Cannabinoids; Cav3.2 channel; Dorsal horn; Dorsal root ganglia; Dronabinol - pharmacology; Dronabinol - therapeutic use; Female; Hyperalgesia; Hyperalgesia - complications; Hyperalgesia - drug therapy; Inflammation; Laboratories; Male; Mice; Micro Report; Pain; Pain - drug therapy; Pain perception; Receptors, Cannabinoid; Sex differences; Spinal cord; Spinal Cord Dorsal Horn; Tetrahydrocannabinol; THC; Δ9-THC; Italy; Analgesia; Pain; Cav3.2 channel; Δ9-THC; Cannabinoid receptors
• ISSN: 1756-6606; 1756-6606
• DOI: 10.1186/s13041-023-01036-8

Delta-9-tetrahydrocannabinol (Δ -THC) is known to produce systemic analgesia that involves CB and CB cannabinoid receptors. However, there is compelling evidence that Δ -THC can potently inhibit Cav3.2T-type calcium channels which are highly expressed in dorsal root ganglion neurons and in the dorsal horn of the spinal cord. Here, we investigated whether spinal analgesia produced by Δ -THC involves Cav3.2 channels vis a vis cannabinoid receptors. We show that spinally delivered Δ -THC produced dose-dependent and long-lasting mechanical anti-hyperalgesia in neuropathic mice, and showed potent analgesic effects in models of inflammatory pain induced by formalin or Complete Freund's Adjuvant (CFA) injection into the hind paw, with the latter showing no overt sex differences. The Δ -THC mediated reversal of thermal hyperalgesia in the CFA model was abolished in Cav3.2 null mice, but was unaltered in CB and CB null animals. Hence, the analgesic effects of spinally delivered Δ -THC are due to an action on T-type calcium channels, rather than activation of spinal cannabinoid receptors.