Telomeric Noncoding RNA TERRA Is Induced by Telomere Shortening to Nucleate Telomerase Molecules at Short Telomeres

Elongation of a short telomere depends on the action of multiple telomerase molecules, which are visible as telomerase RNA foci or clusters associated with telomeres in yeast and mammalian cells. How several telomerase molecules act on a single short telomere is unknown. Herein, we report that the t...

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Bibliographic Details
Published inMolecular cell Vol. 51; no. 6; pp. 780 - 791
Main Authors Cusanelli, Emilio, Romero, Carmina Angelica Perez, Chartrand, Pascal
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 26.09.2013
Subjects
DNA Replication - genetics
image analysis
In Situ Hybridization, Fluorescence
interphase
mammals
non-coding RNA
RNA, Untranslated - genetics
RNA, Untranslated - metabolism
RNA, Untranslated - ultrastructure
S Phase - genetics
Saccharomyces cerevisiae - genetics
telomerase
Telomerase - genetics
Telomerase - metabolism
Telomere - genetics
Telomere - ultrastructure
Telomere Shortening - genetics
telomeres
yeasts
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Summary:Elongation of a short telomere depends on the action of multiple telomerase molecules, which are visible as telomerase RNA foci or clusters associated with telomeres in yeast and mammalian cells. How several telomerase molecules act on a single short telomere is unknown. Herein, we report that the telomeric noncoding RNA TERRA is involved in the nucleation of telomerase molecules into clusters prior to their recruitment at a short telomere. We find that telomere shortening induces TERRA expression, leading to the accumulation of TERRA molecules into a nuclear focus. Simultaneous time-lapse imaging of telomerase RNA and TERRA reveals spontaneous events of telomerase nucleation on TERRA foci in early S phase, generating TERRA-telomerase clusters. This cluster is subsequently recruited to the short telomere from which TERRA transcripts originate during S phase. We propose that telomere shortening induces noncoding RNA expression to coordinate the recruitment and activity of telomerase molecules at short telomeres. [Display omitted] •TERRA transcripts are expressed from short telomeres•TERRA expressed from a single telomere accumulate into a perinuclear focus•A TERRA focus is a site of telomerase clustering•A TERRA-telomerase complex localizes to TERRA’s telomere of origin during S phase
Bibliography:http://dx.doi.org/10.1016/j.molcel.2013.08.029
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ISSN:1097-2765
1097-4164
1097-4164
DOI:10.1016/j.molcel.2013.08.029