A garlic substance disrupts odorant-binding protein recognition of insect pheromones released from adults of the angoumois grain moth, Sitotroga cerealella (Lepidoptera: Gelechiidae)

• Published in: Insect molecular biology Vol. 25; no. 5; pp. 530 - 540
• Main Authors: Ma, M., Chang, M.-M., Lei, C.-L., Yang, F.-L.
• Format: Journal Article
• Language: English
• Published: England Blackwell Publishing Ltd 01.10.2016
• Subjects: Allium sativum; Allyl Compounds - metabolism; Animals; Arthropod Antennae - metabolism; Base Sequence; Carrier Proteins - genetics; Carrier Proteins - metabolism; Cloning, Molecular; DNA, Complementary - genetics; DNA, Complementary - metabolism; Female; Garlic - chemistry; garlic active substance; Gelechiidae; Insect Proteins - genetics; Insect Proteins - metabolism; Lepidoptera; Male; mating disruption; Moths - genetics; Moths - metabolism; odorant-binding protein; olfaction; Phylogeny; Real-Time Polymerase Chain Reaction; Receptors, Odorant - genetics; Receptors, Odorant - metabolism; Sex Attractants - metabolism; Sitotroga cerealella; Sulfides - metabolism; Sitotroga cerealella; odorant-binding protein; olfaction; garlic active substance; mating disruption
• ISSN: 0962-1075; 1365-2583
• DOI: 10.1111/imb.12240

The angoumois grain moth, Sitotroga cerealella, is one of the most serious stored‐grain pests worldwide. Control of this moth may be achieved by interfering with olfactory pathways to disrupt male–female communication with sex pheromones, using plant volatiles like garlic or its active substances. Here, three odorant‐binding protein (OBP) genes [namely Si. cerealella general OBP 1 (ScerGOBP1), ScerGOBP2 and Si. cerealella pheromone‐binding protein (ScerPBP)] were cloned from Si. cerealella antennae, and quantitative real‐time PCR showed that these genes were predominantly expressed in adult antennae. ScerPBP expression was male‐biased, but ScerGOBP1 and ScerGOBP2 were similar between sexes. The results of competitive binding assays indicated that a garlic substance, diallyl trisulphide (DATS), had similar or even higher binding affinity to ScerPBP than Si. cerealella sex pheromone, 7Z, 11E‐hexadecadien‐1‐ol acetate (HDA). In olfactometer bioassays, DATS significantly reduced the response of adults to HDA when they were exposed to air filled with HDA and DATS. Surprisingly, ScerGOBP2, which is postulated to be involved in the detection of general odours, displayed higher affinity with HDA than did ScerPBP, indicating that ScerGOBP2 may also have a role in pheromone perception. These data suggest that DATS may interfere with recognition of female‐produced sex pheromone, disrupting female and male mating behaviour and resulting in a new idea for controlling stored grain pests.