水溶性ZnSe量子点在快速灵敏检测牛分枝杆菌表面MPB83蛋白中的应用

本实验以巯基丙酸作为稳定剂,在水相条件下快速合成稳定性好的水溶性硒化锌量子点（ ZnSe QDs）,采用透射电子显微镜、X射线衍射、红外光谱、荧光以及紫外光谱法等对ZnSe QDs进行了材料表征。将得到的ZnSe QDs通过共价结合方式标记到牛分枝杆菌表面的MPB83蛋白抗体分子上,基于抗体与MPB83蛋白的特异性相互作用,建立了一种检测MPB83蛋白的光谱新方法。研究了pH值及温度对检测的影响,得到pH 8.5,37℃为适宜的体系酸度和温度。在优化的实验条件下,MPB83蛋白浓度在44~528 mg/L范围内,QDs的荧光强度与蛋白浓度间呈现良好的线性关系,此方法对MPB83蛋白的检出限为4...

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Bibliographic Details
Published in分析化学 Vol. 42; no. 5; pp. 643 - 647
Main Author 阮晓娟 王蓓蓓 马美湖 郭爱珍 蔡朝霞
Format Journal Article
LanguageChinese
Published 华中农业大学食品科学技术学院,武汉,430070%华中农业大学动物医学院,武汉,430070 2014
Subjects
MPB83蛋白
ZnSe量子点
牛分枝杆菌
牛结核病
牛结核病
Zinc selenide quantum dots
Mycobacterium bovis
ZnSe量子点
Bovine tuberculosis
牛分枝杆菌
MPB83蛋白
MPB83 protein
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ISSN0253-3820
DOI10.3724/SP.J.1096.2014.31216

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Summary:本实验以巯基丙酸作为稳定剂,在水相条件下快速合成稳定性好的水溶性硒化锌量子点（ ZnSe QDs）,采用透射电子显微镜、X射线衍射、红外光谱、荧光以及紫外光谱法等对ZnSe QDs进行了材料表征。将得到的ZnSe QDs通过共价结合方式标记到牛分枝杆菌表面的MPB83蛋白抗体分子上,基于抗体与MPB83蛋白的特异性相互作用,建立了一种检测MPB83蛋白的光谱新方法。研究了pH值及温度对检测的影响,得到pH 8.5,37℃为适宜的体系酸度和温度。在优化的实验条件下,MPB83蛋白浓度在44~528 mg/L范围内,QDs的荧光强度与蛋白浓度间呈现良好的线性关系,此方法对MPB83蛋白的检出限为4.4 mg/L。该方法为实现准确而及时的诊断牛结核病提供一定理论依据。
Bibliography:22-1125/O6
RUAN Xiao-Juan, WANG Bei-Bei, MA Mei-Hu, GUO Ai-Zhen, CAI Zhao-Xia 1 （ Food Science and Technology College, Huazhong Agricultural University, Wuhan 430070, China） 2 （ College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China）
Zinc selenide quantum dots;Bovine tuberculosis;Mycobacterium bovis;MPB83 protein
A basic water phase method was used for synthesizing water-soluble ZnSe quantum dots （ QDs ） with 3-mercaptopropionic acid （ MPA ） as ligands. The obtained MPA-ZnSe QDs were characterized by transmission electron microscopy, X-ray powder diffraction spectrometry, ultraviolet-visible spectrometry and fluorescence spectrometry. Then the prepared MPA-ZnSe QDs were conjugated with anti-MPB83 antibodies to form the fluorescent probe. As a result of specific interaction, MPB83 proteins were selectively captured by immunized MPA-ZnSe QDs which led to the change of a fluorescent signal. Based on this, a fluorescence-linked immunoassay method was demonstrated for the detection of M
ISSN:0253-3820
DOI:10.3724/SP.J.1096.2014.31216