高速逆流色谱制备分离紫甘薯花色苷

采用高速逆流色谱法分离纯化紫甘薯花色苷。以正丁醇-乙酸乙酯-0.5%乙酸（3∶1∶4,V/V）为溶剂体系,上相为固定相,下相为流动相,流速2 mL/min,进样量300 mg,分离得到两种花色苷的混合物;混合物再以0.2%三氟乙酸-正丁醇-甲基叔丁基醚-乙腈（6∶5∶2∶1,V/V）为溶剂体系,上相为固定相,下相为流动相,流速1.5 mL/min,进样量100 mg,分离得到纯度分别为98.5%和96.7%的两个花色苷单体组分。通过紫外-可见光谱、质谱、核磁等技术进行结构鉴定,确定组分1为3-O-{6-O-（E）-咖啡酰-2-O-[6-O-（E）-对羟基苯甲酰-β-D-吡喃葡糖基]-β-D-吡...

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Bibliographic Details
Published in分析化学 Vol. 39; no. 6; pp. 851 - 856
Main Author 陆英 李佳银 罗晋 李觅路 刘仲华
Format Journal Article
LanguageChinese
Published 国家植物功能成分利用工程技术研究中心,长沙,410128 2011
湖南省作物种质创新与资源利用重点实验室,长沙,410128%湖南农业大学园艺园林学院,长沙,410128
湖南农业大学园艺园林学院,长沙,410128
Subjects
制备分离
紫甘薯
花色苷
高速逆流色谱
制备分离
花色苷
紫甘薯
高速逆流色谱
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ISSN0253-3820
DOI10.3724/SP.J.1096.2011.00851

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Summary:采用高速逆流色谱法分离纯化紫甘薯花色苷。以正丁醇-乙酸乙酯-0.5%乙酸（3∶1∶4,V/V）为溶剂体系,上相为固定相,下相为流动相,流速2 mL/min,进样量300 mg,分离得到两种花色苷的混合物;混合物再以0.2%三氟乙酸-正丁醇-甲基叔丁基醚-乙腈（6∶5∶2∶1,V/V）为溶剂体系,上相为固定相,下相为流动相,流速1.5 mL/min,进样量100 mg,分离得到纯度分别为98.5%和96.7%的两个花色苷单体组分。通过紫外-可见光谱、质谱、核磁等技术进行结构鉴定,确定组分1为3-O-{6-O-（E）-咖啡酰-2-O-[6-O-（E）-对羟基苯甲酰-β-D-吡喃葡糖基]-β-D-吡喃葡糖苷}-[5-O-（β-D-吡喃葡糖苷）]芍药素,组分2为3-O-{6-O-（E）-咖啡酰-2-O-[6-O-（E）-阿魏酰-β-D-吡喃葡糖基]-β-D-吡喃葡糖苷}-[5-O-（β-D-吡喃葡糖苷）]芍药素。实验从450 g紫甘薯中获得63 mg组分1和48 mg组分2,为紫甘薯花色苷分离提供了高效、稳定、可靠的方法。
Bibliography:22-1125/O6
LU Ying,LI Jia-Yin,LUO Jin,LI Mi-Lu,LIU Zhong-Hua（National research Center of Engineering Technology For Utilization of Functional Ingredients From Botanicals,Changsha 410128） 2（Department of Botanical Resource Engineering, College of Horticulture and Landscape,Hunan Agricultural University,Changsha 410128） 3（Hunan Province Key Laboratory of Crop Germplasm Innovation and Utilization,Changsha 410128）
Purple Sweet Potato; Anthocyanins; High-speed Counter-current Chromatography; Preparative Isolation
High-speed counter-current chromatography（HSCCC） was successfully applied to separate and purify anthocyanins from purple sweet potato.With a two-phase solvent system composed of n-butanol-ethylacetate-0.5% acetic acid（3∶1∶4,V/V）,the upper phase as stationary phase,the lower phase as mobile phase at a flow rate of 2.0 mL/min,and injection volume of 300 mg,a mixgure of two anthocyanins was received by undertaking HSCCC.Subsequently,a refining HSCCC was performed to separate and purify these two compounds,with
ISSN:0253-3820
DOI:10.3724/SP.J.1096.2011.00851