Troponin I Switching in the Developing Heart

Monoclonal antibodies identify two distinct isoforms of troponin I in rat cardiac muscle, one predominant in the embryonic and fetal heart and one predominant in the adult heart. The two isoforms can be resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, with apparent molecular we...

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Bibliographic Details
Published inThe Journal of biological chemistry Vol. 264; no. 27; pp. 16299 - 16302
Main Authors Saggin, L, Gorza, L, Ausoni, S, Schiaffino, S
Format Journal Article
LanguageEnglish
Published Bethesda, MD Elsevier Inc 25.09.1989
American Society for Biochemistry and Molecular Biology
Subjects
Aging
Analytical, structural and metabolic biochemistry
Animals
Animals, Newborn
Antibodies, Monoclonal
Biological and medical sciences
Cattle
Contractile proteins
Fetus
Fundamental and applied biological sciences. Psychology
heart
Heart - growth & development
Heart Atria - growth & development
Heart Ventricles - growth & development
Holoproteins
Immunoblotting
Proteins
Rats
Troponin - analysis
Troponin I
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Summary:Monoclonal antibodies identify two distinct isoforms of troponin I in rat cardiac muscle, one predominant in the embryonic and fetal heart and one predominant in the adult heart. The two isoforms can be resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, with apparent molecular weights of 27,000 and 31,500, respectively. The adult isoform is specifically recognized by a monoclonal antibody that is unreactive with the embryonic variant, while two other monoclonal antibodies recognize both isoforms. A monoclonal antibody to cardiac troponin T was used to isolate by affinity chromatography the troponin complex from adult and neonatal rat heart. Affinity purified troponin from neonatal heart was found to contain both the embryonic and adult isoforms of troponin I. Comparative immunoblotting analysis with different muscle tissues shows that embryonic troponin I is identical with respect to electrophoretic mobility and pattern of immunoreactivity to the major troponin I isoform found in adult slow skeletal muscle. Troponin I switching may be implicated in developmental changes involving Ca2+ and pH sensitivity of the contractile system and response to β-adrenergic stimulation.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:0021-9258
1083-351X
DOI:10.1016/S0021-9258(18)71621-9