A Simple and User-Friendly Method for High-Quality Preparation of Pollen Grains for Scanning Electron Microscopy (SEM)

• Published in: Plants (Basel) Vol. 13; no. 15; p. 2140
• Main Authors: Ermolaev, Aleksey, Mardini, Majd, Buravkov, Sergey, Kudryavtseva, Natalya, Khrustaleva, Ludmila
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 01.08.2024
• Subjects: Alcohol; Analysis; Apertures; beets; biochemical compounds; Biomolecules; Carbon dioxide; Chemical tests and reagents; Comparative analysis; corn; Critical point; Dehydration; Dehydration (Physiology); dicotyledons; Drying; Electron microscopy; Environmental monitoring; Equipment costs; folding/unfolding pathways; Formaldehyde; Genetic engineering; Genetically modified organisms; Grain; hexamethyldisilazane; Hydration; insect pollination; Insects; light microscopes; Liliopsida; Lipids; Methods; Morphology; onions; Palynology; Plant breeding; Pollen; pollen grains; quantitative analysis; radishes; Reagents; rye; Scanning electron microscopy; SEM; shrinkage; species; theoretical models; Tobacco; Tomatoes; wheat; Russia; pollen grains; hexamethyldisilazane; dicotyledons; monocotyledons; SEM; folding/unfolding pathways; shrinkage

Pollen is becoming an increasingly important subject for molecular researchers in genetic engineering, plant breeding, and environmental monitoring. To broaden the scope of these studies, it is essential to develop accessible methods for scientists who are not specialized in palynology. The article...