Therapeutic effect of platelet-rich plasma on glucocorticoid-induced rat bone marrow mesenchymal stem cells in vitro

• Published in: BMC musculoskeletal disorders Vol. 23; no. 1; p. 151
• Main Authors: Wang, Yanxue, Luan, Shuo, Yuan, Ze, Lin, Caina, Fan, Shengnuo, Wang, Shaoling, Ma, Chao, Wu, Shaoling
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 15.02.2022; BioMed Central; BMC
• Subjects: Alkaline phosphatase; Animals; Apoptosis; Bcl-2 protein; Blood platelets; Bone growth; Bone marrow; Bone Marrow Cells; Bone marrow mesenchymal stem cells; Bone regeneration; Bones; Care and treatment; Caspase-3; Cbfa-1 protein; Cell cycle; Cell Differentiation; Cell growth; Cell migration; Cell proliferation; Cells, Cultured; Cholecystokinin; Complications and side effects; Corticosteroids; Dexamethasone; Flow cytometry; Glucocorticoid; Glucocorticoids; Glucocorticoids - toxicity; Growth factors; Health aspects; Hematopoietic stem cells; Joint diseases; Laboratory animals; Mesenchymal Stem Cells; Musculoskeletal diseases; Necrosis; Osteogenesis; Osteonecrosis; Plasma; Platelet-Rich Plasma; Platelets; Rats; Regeneration; Stem cell transplantation; Stem cells; Therapeutics, Experimental; China; Beijing China; United States > US; Bone marrow mesenchymal stem cells; Glucocorticoid; Platelet-rich plasma
• ISSN: 1471-2474; 1471-2474
• DOI: 10.1186/s12891-022-05094-2

Glucocorticoid-induced osteonecrosis of the femoral head (GIONFH) is a progressive and disabling disease caused by long-term or high-dose glucocorticoid use. Decreased osteogenesis and proliferation of bone marrow mesenchymal stem cells (BMSCs) are the main pathogenesis of GIONFH. Platelet-rich plasma (PRP) has been shown to play a promising role in bone regeneration. However, the effects of PRP on glucocorticoid-induced BMSCs inhibition remains elusive. The objective of this study was to explore whether PRP could improve the in vitro biological activities of BMSCs inhibited by high-dose glucocorticoid in vitro. In this study, a dexamethasone (Dex)-induced in vitro cell model was established. The effects of PRP on proliferation, migration, cell cycle and apoptosis of rat BMSCs induced with high-dose Dex compared to BMSC , using CCK-8 assay, transwell, flow cytometry and TUNEL assay, respectively. We further performed the alkaline phosphatase (ALP) and alizarin red (ALR) staining to explore the influence of PRP on osteogenic differentiation. Western Blot was used to detect the expression of Bcl-2, Caspase-3, RUNX2 apoptosis, and osteogenic-related proteins. We observed increased apoptosis rate and Caspase-3 expression, and the decreased migration and osteogenic differentiation, and down-regulation of RUNX-2 and Bcl-2 expression in Dex-induced BMSCs. PRP could reverse these inhibitory effects of Dex, and enhance the BMSCs proliferation, migration, and osteogenic ability in vitro. Our vitro study showed that PRP significantly protected BMSCs from Dex-induced apoptosis, and further promoted BMSCs proliferation, migration, and osteogenic differentiation. This study provides a scientific basis for the prevention and treatment of GIONFH with PRP. Meanwhile, it also lays the foundation for the application of PRP in other musculoskeletal diseases.