Laminin-1-induced migration of multiple myeloma cells involves the high-affinity 67 kD laminin receptor

• Published in: British journal of cancer Vol. 85; no. 9; pp. 1387 - 1395
• Main Authors: VANDE BROEK, I, VANDERKERKEN, K, DE GREEF, C, ASOSINGH, K, STRAETMANS, N, VAN CAMP, B, VAN RIET, I
• Format: Journal Article
• Language: English
• Published: Basingstoke Nature Publishing Group 02.11.2001
• Subjects: Animals; Antigens; Biological and medical sciences; Bone marrow; Bone Marrow Cells; Cancer research; Cell adhesion & migration; Cell Movement; Chemotactic Factors; Cloning; Gene Expression Regulation, Neoplastic; Hematologic and hematopoietic diseases; Hematology; Humans; Laminin - pharmacology; Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis; Magnetic fields; Medical research; Medical sciences; Metastasis; Mice; Multiple myeloma; Multiple Myeloma - pathology; Neoplastic Cells, Circulating; Peptides; Proteins; Receptors, Laminin - physiology; Regular; Tumor Cells, Cultured; Belgium; Human; Multiple; Migration; Malignant tumor; Metastasis; Myeloma; Chemotaxis; In vitro; Mechanism; Basement membrane; Cell motility; Laminin; Animal; Extravasation; Established cell line; Affinity; Tumor cell; Biological receptor
• ISSN: 0007-0920; 1532-1827
• DOI: 10.1054/bjoc.2001.2078

The 67 kD laminin receptor (67LR) binds laminin-1 (LN), major component of the basement membrane, with high affinity. In this study, we demonstrated that human multiple myeloma cell lines (HMCL) and murine 5T2MM cells express 67LR. CD38(bright+) plasma cells in fresh multiple myeloma (MM) bone marrow (BM) samples showed weaker 67LR expression, but expression increased after direct exposure to a BM endothelial cell line (4LHBMEC). LN stimulated the in vitro migration of 3 HMCL (MM5.1, U266 and MMS.1), primary MM cells and the murine 5T2MM cells. 67LR has been shown to mediate the actions of LN through binding to CDPGYIGSR, a 9 amino acid sequence from the B1 chain of LN. MM cell migration was partially blocked by peptide 11, a synthetic nonapeptide derived from this amino sequence and also by a blocking antiserum against 67LR. Co-injection of peptide 11 with 5T2MM cells in a murine in vivo model of MM resulted in a decreased homing of 5T2MM cells to the BM compartment. In conclusion, LN acts as a chemoattractant for MM cells by interaction with 67LR. This interaction might be important during extravasation of circulating MM cells.