Molecular characterization and antiviral effects of canine interferon regulatory factor 1 (CaIRF1)

• Published in: BMC veterinary research Vol. 18; no. 1; p. 440
• Main Authors: Hao, Xiangqi, Chen, Hui, Li, Yanchao, Chen, Bo, Liang, Weifeng, Xiao, Xiangyu, Zhou, Pei, Li, Shoujun
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 16.12.2022; BioMed Central; BMC
• Subjects: Analysis; Animals; Antiviral activity; Antiviral agents; Antiviral Agents - pharmacology; Antiviral characterization; Bioinformatics; Canine interferon regulatory factor 1; Cloning; CPV-2; Dogs; Gene expression; Gene Expression Regulation; Health aspects; Immune response; Immunofluorescence; Interferon; Interferon regulatory factor; Interferon regulatory factor 1; Interferon Regulatory Factor-1 - genetics; Interferon Regulatory Factor-1 - metabolism; Interferon-beta - genetics; Interferons - metabolism; ISGs; ISRE; Localization; Mammals; Parvoviruses; Phylogenetics; Protein structure; Proteins; Signal transduction; Stomatitis; Structure; Testing; Transcription factors; Viral infections; Viruses; β-Interferon; China; Antiviral characterization; CPV-2; ISGs; Canine interferon regulatory factor 1; ISRE
• ISSN: 1746-6148; 1746-6148
• DOI: 10.1186/s12917-022-03539-3

Interferon regulatory factor 1 (IRF1) is an important transcription factor that activates the type I interferon (IFN-I) response and plays a vital role in the antiviral immune response. Although IRF1 has been identified in several mammals, little information related to its function in canines has been described. In this study, canine IRF1 (CaIRF1) was cloned. After a series of bioinformatics analyses, we found that the CaIRF1 protein structure was similar to that of other animal IRF1 proteins, including a conserved DNA-binding domain (DBD), an IRF-association domain 2 (IAD2) domain and two nuclear localization signals (NLSs). An indirect immunofluorescence assay (IFA) revealed that CaIRF1 was mainly distributed in the nucleus. Overexpression of CaIRF1 in Madin-Darby canine kidney cells (MDCK) induced high levels of interferon β (IFNβ) and IFN-stimulated response element (ISRE) promoter activation and induced interferon-stimulated gene (ISG) expression. Subsequently, we assayed the antiviral activity of CaIRF1 against vesicular stomatitis virus (VSV) and canine parvovirus type-2 (CPV-2) in MDCK cells. Overexpression of CaIRF1 effectively inhibited the viral yields of VSV and CPV-2, while knocking down of CaIRF1 expression mildly increased viral gene copies. CaIRF1 is involved in the cellular IFN-I signaling pathway and plays an important role in the antiviral response.