Synthetic Peptides Derived from Bovine Lactoferricin Exhibit Antimicrobial Activity against E. coli ATCC 11775, S. maltophilia ATCC 13636 and S. enteritidis ATCC 13076

• Published in: Molecules (Basel, Switzerland) Vol. 22; no. 3; p. 452
• Main Authors: Huertas Méndez, Nataly De Jesús, Vargas Casanova, Yerly, Gómez Chimbi, Anyelith Katherine, Hernández, Edith, Leal Castro, Aura Lucia, Melo Diaz, Javier Mauricio, Rivera Monroy, Zuly Jenny, García Castañeda, Javier Eduardo
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 12.03.2017; MDPI
• Subjects: Amino Acid Sequence; Animals; Anti-Bacterial Agents - chemical synthesis; Anti-Bacterial Agents - pharmacology; Antibacterial activity; Antibacterial agents; Antimicrobial activity; Cattle; Circular Dichroism; Dichroism; E coli; Eggs; Escherichia coli; Escherichia coli - drug effects; High-performance liquid chromatography; Lactoferricin B; Lactoferrin - chemistry; Liquid chromatography; Mass spectrometry; Mass spectroscopy; Microbial Sensitivity Tests; Minimum inhibitory concentration; Molecular Structure; Peptides; Peptides - chemical synthesis; Peptides - pharmacology; Poultry; S. enteritidis; S. maltophilia; Salmonella; Salmonella enteritidis - drug effects; Synthetic peptides; antibacterial activity; Lactoferricin B; S. maltophilia; synthetic peptides; S. enteritidis; E. coli
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules22030452

Linear, dimeric, tetrameric, and cyclic peptides derived from lactoferricin B-containing non-natural amino acids and the RWQWR motif were synthesized, purified, and characterized using RP-HPLC, MALDI-TOF mass spectrometry, and circular dichroism. The antibacterial activity of peptides against ATCC 11775, ATCC 13636, and ATCC 13076 was evaluated. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined. The synthetic bovine lactoferricin exhibited antibacterial activity against ATCC 11775 and ATCC 13076. The dimeric peptide (RRWQWR)₂K-Ahx exhibited the highest antibacterial activity against the tested bacterial strain. The monomeric, cyclic, tetrameric, and palindromic peptides containing the RWQWR motif exhibited high and specific activity against ATCC 11775. The results suggest that short peptides derived from lactoferricin B could be considered as potential candidates for the development of antibacterial agents against infections caused by .