Changes of ECM and CAM gene expression profile in the cirrhotic liver after HCV infection： Analysis by cDNA expression array

• Published in: World journal of gastroenterology : WJG Vol. 11; no. 14; pp. 2184 - 2187
• Main Authors: Xu, Xin, Li, Yi-Ming, Ji, Hong, Hou, Chong-Zhi, Cheng, Ying-Bo, Ma, Fu-Ping
• Format: Journal Article
• Language: English
• Published: United States Department of General Surgery, Second Hospital of Xi'an Jiaotong University, Xi'an 710004, Shaanxi Province, China%Department of General Surgery, Second Hospital of Xianyang, Xianyang 721000, Shaanxi Province, China 14.04.2005; Baishideng Publishing Group Inc
• Subjects: Adult; Brief Reports; CAM; cDNA; Cell Adhesion Molecules - genetics; Down-Regulation - genetics; ECM; Extracellular Matrix - physiology; Female; Gene Expression Profiling; Hepatitis C, Chronic - genetics; Humans; Liver - physiology; Liver Cirrhosis - genetics; Liver Cirrhosis - virology; Male; Middle Aged; Oligonucleotide Array Sequence Analysis; Up-Regulation - genetics; 丙型肝炎; 细胞外表达; 细胞支持分子; 肝硬化; Extracellular matrix; mRNA array analysis; Hepatitis C virus; Cellular adhesion molecules; Liver cirrhosis
• ISSN: 1007-9327; 2219-2840
• DOI: 10.3748/wjg.v11.i14.2184

AIM: We aimed to observe the expression of extracellular matrix (ECM) and cellular adhesion molecules (CAM) in cirrhotic liver tissues after hepatitis C virus (HCV) infection.METHODS: Twelve patients with post HCV inflammatory liver cirrhosis were selected to evaluate their liver function and other virological, pathological parameters, Then three specimens of cirrhotic patients whose health assessment results and laboratory data were similar and three normal liver specimens explanted from liver grafts prepared for liver transplantation were chosen for investigating gene expression of ECM and CAM using cDNA expression array,RESULTS: The cDNA array assay revealed 36.7% (36/96)of genes with changes, in which 26.3% (26/96) was upregulated and 10.1% (10/96) was down-regulated. Integrin (ITGA), collagen (COL), ADAMTS were identified as the characteristic changes of ECM and CAM gene expression levels. ITGA were demonstrated β1 and β2 sub-section changed in liver cirrhosis.CONCLUSION: ECM and CAM play an important role in the progression of liver cirrhosis after HCV infection. The capital mechanism is related to the inflammatory cells infiltration, the activation and transformation of ECM producing cells and the imbalance between production and elimination of ECM.