Utilization of concentrate after membrane filtration of sugar beet thin juice for ethanol production

• Published in: Bioresource technology Vol. 133; pp. 134 - 141
• Main Authors: Kawa-Rygielska, Joanna, Pietrzak, Witold, Regiec, Piotr, Stencel, Piotr
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 01.04.2013; Elsevier
• Subjects: batch fermentation; Beta vulgaris; Beta vulgaris - chemistry; Beverages; Biological and medical sciences; Biotechnology; Carbohydrate Metabolism; Carbohydrate Metabolism - drug effects; chemistry; Concentrate; Culture Media; Culture Media - pharmacology; drug effects; Ethanol; Ethanol - metabolism; Ethanol fermentation; ethanol production; Ethyl alcohol; Fermentation; Fermentation - drug effects; Fundamental and applied biological sciences. Psychology; juice concentrates; Juices; Kinetics; Media; Membrane filtration; Membranes, Artificial; metabolism; methods; Methods. Procedures. Technologies; Microbial engineering. Fermentation and microbial culture technology; Microbial Viability; Microbial Viability - drug effects; nutrients; pharmacology; Saccharomyces cerevisiae; Saccharomyces cerevisiae - drug effects; Saccharomyces cerevisiae - metabolism; salts; Strain; sugar beet; Sugar beet juice; Sugar beets; sugars; ultrafiltration; Ultrafiltration - methods; Yeast; yeast extract; yeasts; Sugar beet juice; Ethanol fermentation; Concentrate; Saccharomyces cerevisiae; Membrane filtration; Ascomycota; Fungi; Ethanol; Alcoholic fermentation; Sugar beet; Sugar juice
• ISSN: 0960-8524; 1873-2976; 1873-2976
• DOI: 10.1016/j.biortech.2013.01.070

► Concentrate after ultrafiltration of sugar beet thin juice as ethanol production feed. ► Two yeast strains and three supplementation variants tested for process improvement. ► Fermentation of the concentrate ended with ca. 82% ethanol yield. ► Nutrient supplementation further improved ethanol yield. The subject of this study was to investigate the feasibility of the concentrate obtained after membrane ultrafiltration of sugar beet thin juice for ethanol production and selection of fermentation conditions (yeast strain and media supplementation). Resulting concentrate was subjected to batch ethanol fermentation using two strains of Saccharomyces cerevisiae (Ethanol Red and Safdistill C-70). The effect of different forms of media supplementation (mineral salts: (NH4)2SO4, K2HPO4, MgCl2; urea+Mg3(PO4)2 and yeast extract) on the fermentation course was also studied. It was stated that sugar beet juice concentrate is suitable for ethanol production yielding, depending on the yeast strain, ca. 85–87gL−1 ethanol with ca. 82% practical yield and more than 95% of sugars consumption after 72h of fermentation. Nutrients enrichment further increased ethanol yield. The best results were obtained for media supplemented with urea+Mg3(PO4)2 yielding 91.16–92.06gL−1 ethanol with practical yield ranging 84.78–85.62% and full sugars consumption.