Minhee Analysis Package: an integrated software package for detection and management of spontaneous synaptic events

• Published in: Molecular brain Vol. 14; no. 1; p. 138
• Main Authors: Kim, Yong Gyu, Shin, Jae Jin, Kim, Sang Jeong
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 08.09.2021; BioMed Central; BMC
• Subjects: Algorithms; Analysis of Variance; Animals; Brain - physiology; Computer programs; Computer Simulation; Data analysis software; Data Display; Data management; Electrophysiology; Event detection; Hypotheses; Integrated software; Male; Methodology; Mice; Mice, Inbred C57BL; Miniature Postsynaptic Potentials; Models, Neurological; Neurons - physiology; Neurophysiology; Neurophysiology - methods; Postsynaptic current; Pyramidal Cells - physiology; Quantitative analysis; Signal processing; Software; Software packages; Standard deviation; Statistics, Nonparametric; Synapses - physiology; Synaptic Potentials; Synaptic strength; User-Computer Interface; Workflow; United States > US; Electrophysiology; Event detection; Postsynaptic current; Data analysis software; Data management
• ISSN: 1756-6606; 1756-6606
• DOI: 10.1186/s13041-021-00847-x

To understand the information encoded in a connection between the neurons, postsynaptic current (PSC) has been widely measured as a primary index of synaptic strength in the field of neurophysiology. Although several automatic detection methods for PSCs have been proposed to simplify a workflow in the analysis, repetitive steps such as quantification and management of PSC data should be still performed with much effort. Here, we present Minhee Analysis Package, an integrated standalone software package that is capable of detecting, sorting, and quantifying PSC data. First, we developed a stepwise exploratory algorithm to detect PSC and validated our detection algorithm using the simulated and experimental data. We also described all the features and examples of the package so that users can use and follow them properly. In conclusion, our software package is expected to improve the convenience and efficiency of neurophysiologists to analyze PSC data by simplifying the workflow from detection to quantification. Minhee Analysis Package is freely available to download from http://www.github.com/parkgilbong/Minhee_Analysis_Pack .