Serological evaluation for the current epidemic situation of foot and mouth disease among cattle and buffaloes in Egypt

• Published in: Veterinary World Vol. 13; no. 1; pp. 1 - 9
• Main Authors: El-Rhman, Mariam M Abd, Abo El-Hassan, Diea G, Awad, Walid S, Salem, Sayed A H
• Format: Journal Article
• Language: English
• Published: India Veterinary World 01.01.2020
• Subjects: 3abc enzyme-linked immunosorbent assay test; Antibodies; Beef cattle; Cattle; Diseases; Egypt; Enzyme-linked immunosorbent assay; Enzymes; Epidemics; Foot & mouth disease; Foot and mouth disease; foot-and-mouth disease virus; Immune response; Immunogenicity; Immunoglobulins; non-structural protein; Serotypes; Serotyping; Solid phases; solid-phase competitive enzyme-linked immunosorbent assay; Strains (organisms); Vaccination; Vaccine efficacy; Vaccines; Viruses; Egypt; solid-phase competitive enzyme-linked immunosorbent assay; foot-and-mouth disease virus; 3ABC enzyme-linked immunosorbent assay test; non-structural protein; Egypt
• ISSN: 0972-8988; 2231-0916
• DOI: 10.14202/vetworld.2020.1-9

The present study was aimed to investigate the epidemic situation of foot-and-mouth disease (FMD) in Egypt from 2016 to 2018 based on the detection of FMD virus (FMDV) in carrier or previously infected animals, by determination of antibodies against non-structural protein (NSP), implementation a pilot study on circulating FMDV serotypes and assure the efficacy of locally produced inactivated trivalent vaccine. A total of 1500 sera were collected from apparent healthy vaccinated cattle and buffaloes from three Egyptian geographical sectors, representing ten governorates. Determination of FMD antibodies against NSP was carried out using 3ABC enzyme-linked immunosorbent assay (ELISA) test. Serotyping of the circulating FMDV and assure the vaccine efficacy was performed using solid-phase competitive ELISA. The 3ABC ELISA test revealed 26.4% and 23.7% positive for FMDV-NSP antibodies in cattle and buffalo sera, respectively. The highest positivity was in Delta Sector among both cattle 42.3% and buffaloes 28.8%. Serotyping of FMDV-positive NSP sera in El-Qalyubia Governorate for the circulating FMDV serotypes O, A, and Southern African Territories (SAT) 2 was 52.2%, 17.4%, and 30.4% in cattle and 31.8%, 27.3%, and 40.9% in buffaloes, respectively. The overall protection level due to the vaccination program was 62.1 and 60.9% in cattle and buffaloes, respectively, while the protective level of the FMDV serotypes O, A, and SAT2 included in the inactivated trivalent vaccine was 73.9, 84.6, and 63.8% in cattle and 72.3, 82.3, and 63.5% in buffaloes, respectively. The present study recommended full determination for the immunogenic relationship between the vaccine strains and the field strains to attain maximum protection against the circulating viruses.