Enzymatic assay method for measuring mizoribine levels in serum

• Published in: Journal of bioscience and bioengineering Vol. 112; no. 2; pp. 205 - 207
• Main Authors: Hiramitsu, Takahisa, Ota, Hiroko, Watarai, Yoshihiko, Achiha, Masahito, Fukami, Harue, Sakasegawa, Shin-ichi, Hino, Emisa, Yamaguchi, Tamami, Ueda, Shigeru, Kagimoto, Yoshitaka, Tamura, Tomohiro, Uchida, Kazuharu
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01.08.2011; Elsevier
• Subjects: Biological and medical sciences; Biotechnology; blood serum; Chromatography, High Pressure Liquid; Drug monitoring; Enzyme Assays - methods; Fundamental and applied biological sciences. Psychology; high performance liquid chromatography; Humans; Immunosuppressive Agents - blood; Inosine 5′-monophosphate dehydrogenase; Mizoribine; mizoribine 5′-monophosphate; Nucleoside kinase; Regression Analysis; Ribonucleosides - blood; mizoribine 5′-monophosphate; Inosine 5′-monophosphate dehydrogenase; Drug monitoring; Nucleoside kinase; Mizoribine; Drug; mizoribine 5'-monophosphate; Enzyme; Transferases; Dehydrogenase; Enzymatic method; Kinase; Inosine; Nucleoside; Serum; Oxidoreductases; Inosine 5'-monophosphate dehydrogenase; Monitoring
• ISSN: 1389-1723; 1347-4421
• DOI: 10.1016/j.jbiosc.2011.03.017

A sensitive and specific method for assaying serum mizoribine levels that can be applied to general automatic clinical analyzers was developed. Regression analysis of the enzymatic assay ( y) vs. the HPLC method ( x) produced the following relation: y = 0.964 x + 0.090 ( n = 262, Sy, x = 6.37 ng/mL).