Association of serum 25-OH-vitamin D level with FTO and IRX3 genes expression in obese and overweight boys with different FTO rs9930506 genotypes

• Published in: Journal of translational medicine Vol. 19; no. 1; p. 350
• Main Authors: Gholamalizadeh, Maryam, Doaei, Saeid, Mokhtari, Zohreh, Jalili, Vahideh, Bourbour, Fatemeh, Omidi, Saeed, Ebrahimi, Kamal, Kalantari, Naser, Abdi, Sheyda, Azizi Tabesh, Ghasem, Naimi Joubani, Mohammad, Roohbakhsh, Esmaeil, Mosavi Jarrahi, Seyed Alireza
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 16.08.2021; BioMed Central; BMC
• Subjects: 25-OH-vitamin D; Adolescent; Alfacalcidol; Alpha-Ketoglutarate-Dependent Dioxygenase FTO - genetics; Body composition; Body fat; Body weight; Calcifediol; Calories; Children & youth; Development and progression; Exercise; FTO gene; Gene Expression; Gene polymorphism; Genetic aspects; Genotype; Genotype & phenotype; Health aspects; Homeodomain Proteins - genetics; Humans; Iran; IRX3 gene; Male; Musculoskeletal system; Nutrition research; Obesity; Obesity - genetics; Obesity in adolescence; Overweight; Pediatric research; Physical fitness; Polymorphism, Single Nucleotide - genetics; Regression analysis; Risk factors; Single nucleotide polymorphisms; Students; Teenagers; Transcription Factors - genetics; Vitamin D; Vitamin deficiency; Weight control; Iran; South Korea; Obesity; IRX3 gene; FTO gene; 25-OH-vitamin D
• ISSN: 1479-5876; 1479-5876
• DOI: 10.1186/s12967-021-03029-4

The roles of FTO gene and the level of serum 25-OH-vitamin D in obesity are frequently reported. This study aimed to investigate the interactions of serum 25-OH-vitamin D level, FTO and IRX3 genes expression, and FTO genotype in obese and overweight boys. This study was carried out on the 120 male adolescents with overweight in Tehran, Iran. Blood samples were collected from the participants in order to evaluate the serum level of 25-OH-vitamin D, the expression level of FTO and IRX3 genes, and FTO genotype for rs9930506 at baseline and after 18 weeks of the study. In general, no significant association was found between serum 25-OH-vitamin D level and IRX3 and FTO genes expression. The results of linear regression on the relationship between 25-OH-vitamin D serum level and FTO and IRX3 genes expression based on FTO genotypes for rs9930506 indicated that in AA/AG genotype carriers, serum 25-OH-vitamin D level was positively associated with FTO gene expression (B = 0.07, p = 0.02) and inversely associated with IRX3 gene expression (B = - 0.07, p = 0.03). In GG carriers, serum 25-OH-vitamin D level was not associated with expression of IRX3 and FTO genes. There are significant interactions between 25-OH-vitamin D and the expression of FTO and IRX3 genes in the subset of obese patients with specific genotypes for FTO rs9930506. There was no association between serum 25-OH-vitamin D levels and the expression of FTO and IRX genes in individuals with a homozygous genotype for the risk allele of the FTO gene polymorphism.