Vitamin D alleviates oxidative stress in adipose tissue and mesenteric vessels from obese patients with subclinical inflammation

• Published in: Canadian journal of physiology and pharmacology Vol. 98; no. 2; pp. 85 - 92
• Main Authors: Ionica, Mihaela, Aburel, Oana M, Vaduva, Adrian, Petrus, Alexandra, Rațiu, Sonia, Olariu, Sorin, Sturza, Adrian, Muntean, Danina M
• Format: Journal Article
• Language: English
• Published: Canada NRC Research Press 01.02.2020; Canadian Science Publishing NRC Research Press
• Subjects: Adipose tissue; Adipose tissues; Age; Alfacalcidol; C-reactive protein; Calcifediol; Calcitriol; Endothelium; Inflammation; Medical research; Medicine, Experimental; Obesity; Oxidative stress; Pandemics; Patients; Reactive oxygen species; Serum levels; Surgery; Type 2 diabetes; Vitamin D; Romania; artères mésentériques; tissu adipeux viscéral; dysfonctionnement endothélial; mesenteric arteries; endothelial dysfunction; oxidative stress; stress oxydatif; visceral adipose tissue
• ISSN: 0008-4212; 1205-7541
• DOI: 10.1139/cjpp-2019-0340

Obesity is an age-independent, lifestyle-triggered, pandemic disease associated with both endothelial and visceral adipose tissue (VAT) dysfunction leading to cardiometabolic complications mediated via increased oxidative stress and persistent chronic inflammation. The purpose of the present study was to assess the oxidative stress in VAT and vascular samples and the effect of in vitro administration of vitamin D. VAT and mesenteric artery branches were harvested during abdominal surgery performed on patients referred for general surgery ( = 30) that were randomized into two subgroups: nonobese and obese. Serum levels of C-reactive protein (CRP) and vitamin D were measured. Tissue samples were treated or not with the active form of vitamin D: 1,25(OH) D (100 nmol/L, 12 h). The main findings are that in obese patients, ( ) a low vitamin D status was associated with increased inflammatory markers and reactive oxygen species generation in VAT and vascular samples and ( ) in vitro incubation with vitamin D alleviated oxidative stress in VAT and vascular preparations and also improved the vascular function. We report here that the serum level of vitamin D is inversely correlated with the magnitude of oxidative stress in the adipose tissue. Ex vivo treatment with active vitamin D mitigated obesity-related oxidative stress.