RNAi targeting EZH2 inhibits tumor growth and liver metastasis of pancreatic cancer in vivo

• Published in: Cancer letters Vol. 297; no. 1; pp. 109 - 116
• Main Authors: Chen, Yangchao, Xie, Dan, Yin Li, Wing, Man Cheung, Chi, Yao, Hong, Chan, Ching Yu, Chan, Chu-yan, Xu, Fang-Ping, Liu, Yan-Hui, Sung, Joseph J.Y., Kung, Hsiang-fu
• Format: Journal Article
• Language: English
• Published: Ireland Elsevier Ireland Ltd 01.11.2010; Elsevier Limited
• Subjects: Adult; Aged; Animals; Cancer therapies; Carcinoma, Pancreatic Ductal - genetics; Carcinoma, Pancreatic Ductal - metabolism; Carcinoma, Pancreatic Ductal - secondary; Carcinoma, Pancreatic Ductal - therapy; Cell Differentiation; Cell Line, Tumor; Chemotherapy; DNA-Binding Proteins - genetics; DNA-Binding Proteins - metabolism; Enhancer of zeste homolog 2; Enhancer of Zeste Homolog 2 Protein; Female; Genetic Therapy - methods; Hematology, Oncology and Palliative Medicine; Humans; Liver metastasis; Liver Neoplasms - genetics; Liver Neoplasms - metabolism; Liver Neoplasms - prevention & control; Liver Neoplasms - secondary; Male; Mice; Mice, Nude; Middle Aged; Neoplasm Staging; Pancreas; Pancreatic cancer; Pancreatic Neoplasms - genetics; Pancreatic Neoplasms - metabolism; Pancreatic Neoplasms - pathology; Pancreatic Neoplasms - therapy; Polycomb Repressive Complex 2; RNA Interference; Rodents; Studies; Time Factors; Transcription Factors - genetics; Transcription Factors - metabolism; Transfection; Tumor Burden; Tumorigenecity; Up-Regulation; Xenograft Model Antitumor Assays; Enhancer of zeste homolog 2; Pancreatic cancer; Tumorigenecity; Liver metastasis
• ISSN: 0304-3835; 1872-7980; 1872-7980
• DOI: 10.1016/j.canlet.2010.05.003

The function of EZH2 in tumorigenesis and liver metastasis of pancreatic cancer has never been elucidated in vivo. EZH2 was overexpressed in pancreatic carcinomas and its overexpression was associated with tumor differentiation and pT status. Suppression of EZH2 caused a significant growth inhibition of pancreatic cancer cells in vitro and markedly diminished their tumorigenicity in vivo. Knock-down of EZH2 inhibited liver metastasis of pancreatic cancer in vivo. EZH2 has a crucial role in tumor growth and liver metastasis of pancreatic cancer.