Manufacture of chitosan microbeads using centrifugally driven flow of gel-forming solutions through a polymeric micronozzle

• Published in: Journal of colloid and interface science Vol. 336; no. 2; pp. 634 - 641
• Main Authors: Mark, Daniel, Haeberle, Stefan, Zengerle, Roland, Ducree, Jens, Vladisavljević, Goran T.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier Inc 15.08.2009; Elsevier
• Subjects: Beads; Centrifugal; Chemistry; Chitosan; Chitosan - chemistry; Colloidal gels. Colloidal sols; Colloidal state and disperse state; Encapsulation; Exact sciences and technology; General and physical chemistry; Hydrogel microbeads; Ionotropic gelation; Manufactured Materials; Mathematical analysis; Mathematical models; Methods; Microfluidics; Microorganisms; Microspheres; Monodisperse drops; Motors; Nozzles; Particle Size; Physical and chemical studies. Granulometry. Electrokinetic phenomena; Rheology; Rotors; Surface physical chemistry; Encapsulation; Ionotropic gelation; Chitosan; Hydrogel microbeads; Monodisperse drops; Microfluidics; Centrifugal; Experimental data; Gelation; Pulse; Drop; Colloidal gel; Theoretical model; Reproducibility; lonotropic gelation; pH; Frequency; Monodispersed particle; Hydrogel; Diameter; Production process
• ISSN: 0021-9797; 1095-7103
• DOI: 10.1016/j.jcis.2009.04.029

A centrifugally driven pulse-free flow has been used for generation of tripolyphosphate (TPP)-gelated chitosan beads with tunable diameters ranging from 148 to 257 μm. The production process requires a single motor as the sole actively actuated component. The 2% (w/w) chitosan solution was extruded through a polymeric nozzle with an inner diameter of 127 μm in the centrifugal field ranging from 93 to 452 g and the drops were collected in an Eppendorf tube containing 10% (w/w) TPP solution at pH 4.0. The reproducibility of the bead diameters out of different nozzles was very good with overall CVs of the bead diameters down to 15% and the production rate was 45 beads per second per nozzle at 44 Hz rotor frequency. The production rate was proportional to the sixth power of the rotor frequency, which was explained by the non-Newtonian behaviour of the chitosan solution with a flow behaviour index of 0.466. An analytical model for the bead diameter and production rate has been presented and validated by the experimental data. The shrinkage of chitosan drops during gelation was estimated from the observations and the theoretical model.