Cloning and characterization of the dnaKJ operon in Acetobacter aceti

• Published in: Journal of Bioscience and Bioengineering Vol. 97; no. 5; pp. 339 - 342
• Main Authors: Okamoto-Kainuma, Akiko, Yan, Wang, Fukaya, Masahiro, Tukamoto, Yoshinori, Ishikawa, Morio, Koizumi, Yukimichi
• Format: Book Review Journal Article
• Language: English
• Published: Amsterdarm Elsevier B.V 2004; Elsevier Science; Elsevier Limited
• Subjects: acetic acid fermentation; ACETOBACTER; Acetobacter aceti; Biological and medical sciences; Biotechnology; dnaKJ; Fundamental and applied biological sciences. Psychology; GENES; heat-shock protein; MOLECULAR CLONING; Proteobacteria; stress; TRANSCRIPTION; stress; dnaKJ; Acetobacter aceti; heat-shock protein; acetic acid fermentation; Acetobacteraceae; Characterization; Acetic fermentation; Operon; Heat shock protein; Bacteria; Acetic acid; Stress
• ISSN: 1389-1723; 1347-4421
• DOI: 10.1016/S1389-1723(04)70216-9

The dnaKJ operon of Acetobacter aceti was cloned and sequenced. The profile of the gene configuration was similar to that of other α-proteobacteria. In the DnaK and DnaJ proteins of A. aceti, the characteristic domains/motifs reported in other organisms were well conserved. This operon was transcribed in response to a temperature shift and exposure to ethanol/acetic acid. The overexpression of this operon in A. aceti resulted in improved growth compared to the control strain at high temperature or in the presence of ethanol, suggesting a correlation to resistance against stressors present during fermentation, although the overexpression did not increase the resistance to acetic acid.