Shear stress induces expression of CNP gene in human endothelial cells
To elucidate the effect of blood flow on gene transcription of C-type natriuretic peptide (CNP), human umbilical vein endothelial cells were exposed to shear stress in a cone-plate viscometer. Expression of CNP mRNA, evaluated by reverse transcription-polymerase chain reaction, was markedly increase...
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| Published in | FEBS letters Vol. 373; no. 2; pp. 108 - 110 |
|---|---|
| Main Authors | , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
England
Elsevier B.V
09.10.1995
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| Subjects | |
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| Abstract | To elucidate the effect of blood flow on gene transcription of C-type natriuretic peptide (CNP), human umbilical vein endothelial cells were exposed to shear stress in a cone-plate viscometer. Expression of CNP mRNA, evaluated by reverse transcription-polymerase chain reaction, was markedly increased by exposure to shear stress of 24 dyne/cm
2 at 3 h. The CNP mRNA level was maintained until 12 h. Thus, the present study demonstrated for the first time that shear stress induces expression of CNP gene in human endothelial cells. |
|---|---|
| AbstractList | To elucidate the effect of blood flow on gene transcription of C-type natriuretic peptide (CNP), human umbilical vein endothelial cells were exposed to shear stress in a cone-plate viscometer. Expression of CNP mRNA, evaluated by reverse transcription-polymerase chain reaction, was markedly increased by exposure to shear stress of 24 dyne/cm2 at 3 h. The CNP mRNA level was maintained until 12 h. Thus, the present study demonstrated for the first time that shear stress induces expression of CNP gene in human endothelial cells. To elucidate the effect of blood flow on gene transcription of C‐type natriuretic peptide (CNP), human umbilical vein endothelial cells were exposed to shear stress in a cone‐plate viscometer. Expression of CNP mRNA, evaluated by reverse transcription‐polymerase chain reaction, was markedly increased by exposure to shear stress of 24 dyne/cm 2 at 3 h. The CNP mRNA level was maintained until 12 h. Thus, the present study demonstrated for the first time that shear stress induces expression of CNP gene in human endothelial cells. To elucidate the effect of blood flow on gene transcription of C-type natriuretic peptide (CNP), human umbilical vein endothelial cells were exposed to shear stress in a cone-plate viscometer. Expression of CNP mRNA, evaluated by reverse transcription-polymerase chain reaction, was markedly increased by exposure to shear stress of 24 dyne/cm super(2) at 3 h. The CNP mRNA level was maintained until 12 h. Thus, the present study demonstrated for the first time that shear stress induces expression of CNP gene in human endothelial cells. To elucidate the effect of blood flow on gene transcription of C-type natriuretic peptide (CNP), human umbilical vein endothelial cells were exposed to shear stress in a cone-plate viscometer. Expression of CNP mRNA, evaluated by reverse transcription-polymerase chain reaction, was markedly increased by exposure to shear stress of 24 dyne/cm 2 at 3 h. The CNP mRNA level was maintained until 12 h. Thus, the present study demonstrated for the first time that shear stress induces expression of CNP gene in human endothelial cells. |
| Author | Kawasaki, Tomio Fujiwara, Yoshiyuki Ohnishi, Tadashi Monden, Morito Okahara, Kazuhiro Kambayashi, Jun-ichi |
| Author_xml | – sequence: 1 givenname: Kazuhiro surname: Okahara fullname: Okahara, Kazuhiro – sequence: 2 givenname: Jun-ichi surname: Kambayashi fullname: Kambayashi, Jun-ichi – sequence: 3 givenname: Tadashi surname: Ohnishi fullname: Ohnishi, Tadashi – sequence: 4 givenname: Yoshiyuki surname: Fujiwara fullname: Fujiwara, Yoshiyuki – sequence: 5 givenname: Tomio surname: Kawasaki fullname: Kawasaki, Tomio – sequence: 6 givenname: Morito surname: Monden fullname: Monden, Morito |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/7589445$$D View this record in MEDLINE/PubMed |
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| Keywords | PBS cNOS ANP SDS CNP FGF ICAM BNP cGMP mRNA C-type natriuretic peptide Human endothelial cell SSRE RT-PCR FBS Shear stress TGFβ HUVEC GAPDH |
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| SubjectTerms | ANP Atrial Natriuretic Factor - biosynthesis atrium natriuretic peptide Base Sequence BNP brain natriuretic peptide C-type natriuretic peptide Cells, Cultured cGMP cNOS CNP constitutive nitric oxide synthase DNA Primers Endothelium, Vascular - metabolism FBS fetal bovine serum FGF fibroblast growth factor GAPDH Gene Expression glyceraldehyde 3-phosphate dehydrogenase guanosine 3′:5′-cyclic monophosphate Human endothelial cell human umbilical vein endothelial cell Humans HUVEC ICAM intracellular adhesion molecule Molecular Sequence Data mRNA Natriuretic Peptide, C-Type PBS phosphate buffered saline Polymerase Chain Reaction Protein Biosynthesis reverse transcription-polymerase chain reaction RNA, Messenger - analysis RNA, Messenger - biosynthesis RT-PCR SDS Shear stress shear stress responsive element sodium dodecyl sulfate Space life sciences SSRE Stress, Mechanical TGFβ Transcription, Genetic transforming growth factor-beta Umbilical Veins |
| Title | Shear stress induces expression of CNP gene in human endothelial cells |
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