Shear stress induces expression of CNP gene in human endothelial cells

• Published in: FEBS letters Vol. 373; no. 2; pp. 108 - 110
• Main Authors: Okahara, Kazuhiro, Kambayashi, Jun-ichi, Ohnishi, Tadashi, Fujiwara, Yoshiyuki, Kawasaki, Tomio, Monden, Morito
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 09.10.1995
• Subjects: ANP; Atrial Natriuretic Factor - biosynthesis; atrium natriuretic peptide; Base Sequence; BNP; brain natriuretic peptide; C-type natriuretic peptide; Cells, Cultured; cGMP; cNOS; CNP; constitutive nitric oxide synthase; DNA Primers; Endothelium, Vascular - metabolism; FBS; fetal bovine serum; FGF; fibroblast growth factor; GAPDH; Gene Expression; glyceraldehyde 3-phosphate dehydrogenase; guanosine 3′:5′-cyclic monophosphate; Human endothelial cell; human umbilical vein endothelial cell; Humans; HUVEC; ICAM; intracellular adhesion molecule; Molecular Sequence Data; mRNA; Natriuretic Peptide, C-Type; PBS; phosphate buffered saline; Polymerase Chain Reaction; Protein Biosynthesis; reverse transcription-polymerase chain reaction; RNA, Messenger - analysis; RNA, Messenger - biosynthesis; RT-PCR; SDS; Shear stress; shear stress responsive element; sodium dodecyl sulfate; Space life sciences; SSRE; Stress, Mechanical; TGFβ; Transcription, Genetic; transforming growth factor-beta; Umbilical Veins; PBS; cNOS; ANP; SDS; CNP; FGF; ICAM; BNP; cGMP; mRNA; C-type natriuretic peptide; Human endothelial cell; SSRE; RT-PCR; FBS; Shear stress; TGFβ; HUVEC; GAPDH
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/0014-5793(95)01027-C

To elucidate the effect of blood flow on gene transcription of C-type natriuretic peptide (CNP), human umbilical vein endothelial cells were exposed to shear stress in a cone-plate viscometer. Expression of CNP mRNA, evaluated by reverse transcription-polymerase chain reaction, was markedly increased by exposure to shear stress of 24 dyne/cm 2 at 3 h. The CNP mRNA level was maintained until 12 h. Thus, the present study demonstrated for the first time that shear stress induces expression of CNP gene in human endothelial cells.