Effects of ambient ozone exposure on circulating extracellular vehicle microRNA levels in coronary artery disease patients

• Published in: Journal of Toxicology and Environmental Health, Part A Vol. 83; no. 9; pp. 351 - 362
• Main Authors: Chen, Hao, Xu, Yunan, Rappold, Ana, Diaz-Sanchez, David, Tong, Haiyan
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis 02.05.2020; Taylor & Francis Ltd
• Subjects: 2.5; Adult; Aged; Air pollutants; Air pollution; Air pollution effects; Air pollution measurements; beta blocker; Cardiovascular disease; Cardiovascular diseases; Circulating MicroRNA - adverse effects; Circulating MicroRNA - genetics; Coronary artery; Coronary artery disease; Coronary Artery Disease - chemically induced; Coronary Artery Disease - physiopathology; Coronary vessels; Correlation analysis; Diabetes mellitus; Environmental Exposure - adverse effects; Exposure; Female; Gene Expression Regulation - drug effects; Heart diseases; Humans; Male; MicroRNAs; Middle Aged; miRNA; mixed effect model; Morbidity; Ozone; Ozone - adverse effects; Ozone - blood; Particulate emissions; Particulate matter; Pollutants; Pollution effects; repeated measures; Ribonucleic acid; RNA; Signal Transduction - drug effects; Within-subjects design; mixed effect model; Air pollutants; beta blocker; PM2.5; miRNA; ozone; repeated measures
• ISSN: 1528-7394; 1087-2620; 2381-3504
• DOI: 10.1080/15287394.2020.1762814

Exposure to ambient air pollutants such as ozone (O 3 ) and particulate matter (PM) is associated with increased cardiovascular morbidity and rate of mortality, but the underlying biological mechanisms have yet to be described. Emerging evidence shows that extracellular vehicle (EV) microRNAs (miRNAs) may facilitate cell-to-cell and organ-to-organ communications and play a role in the air pollution-induced cardiovascular effects. This study aims to explore the association between air pollutant exposure and miRNA changes related to cardiovascular diseases. Using a panel study design, 14 participants with coronary artery diseases were enrolled in this study. Each participant had up to 10 clinical visits and their plasma samples were collected and measured for expression of miRNA-21 (miR-21), miR-126, miR-146, miR-150, and miR-155. Mixed effects models adjusted for temperature, humidity, and season were used to examine the association between miRNA levels and exposure to 8-hr O 3 or 24-hr PM 2.5 up to 4 days prior. Results demonstrated that miR-150 expression was positively associated with O 3 exposure at 1-4 days lag and 5day moving average while miR-155 expression tracked with O 3 exposure at lag 0. No significant association was found between miRNA expression and ambient PM 2.5 at any time point. β-blocker and diabetic medication usage significantly modified the correlation between O 3 exposure and miR-150 expression where the link was more prominent among non-users. In conclusion, evidence indicated an association between exposure to ambient O 3 and circulating levels of EV miR-150 and miR-155 was observed. These findings pointed to a future research direction involving miRNA-mediated mechanisms of O 3 -induced cardiovascular effects.