Fabrication of a thin-layer solid optical tissue phantom by a spin-coating method: pilot study

• Published in: Journal of biomedical optics Vol. 18; no. 2; p. 025006
• Main Authors: Bae, Yunjin, Son, Taeyoon, Park, Jihoon, Jung, Byungjo
• Format: Journal Article
• Language: English
• Published: United States Society of Photo-Optical Instrumentation Engineers 01.02.2013
• Subjects: Absorption coefficient; Correlation; Epidermis - anatomy & histology; Feasibility; Humans; Multilayers; Optical Imaging; Optical Phenomena; Phantoms, Imaging; Pilots; spin speed; viscosity; concentration; spin coating; solid optical tissue phantom; epidermis
• ISSN: 1083-3668; 1560-2281
• DOI: 10.1117/1.JBO.18.2.025006

Solid optical tissue phantoms (OTPs) have been widely used for many purposes. This study introduces a spin-coating method (SCM) to fabricate a thin-layer solid OTP (TSOTP) with epidermal thickness. TSOTPs are fabricated by controlling the spin speed (250 to 2500 rpm), absorber concentration (0.2% to 1.0%), and the number of layers. The results show that the thicknesses of the TSOTPs are homogeneous in the region of interest. The one-layer TSOTP achieves maximum and minimum thicknesses of 65±0.28  μm (250 rpm) and 5.1±0.17  μm (2500 rpm), respectively, decreasing exponentially as a function of the spin speed. The thicknesses of the multilayer TSOTPs increases as a function of the number of layers and are correlated strongly with the spin speed (R2≥0.95). The concentration of the OTP mixture does not directly affect the thickness of the TSOTP; however, the absorption coefficients exponentially increase as a function of absorber concentration (R2≥0.98). These results suggest that the SCM can be used to fabricate homogeneous TSOTPs with various thicknesses by controlling the spin speed and number of layers. Finally, a double-layer OTP that combines epidermal TSOTP and dermal OTP is manufactured as a preliminary study to investigate the practical feasibility of TSOTPs.