IsaB Inhibits Autophagic Flux to Promote Host Transmission of Methicillin-Resistant Staphylococcus aureus

• Published in: Journal of investigative dermatology Vol. 135; no. 11; pp. 2714 - 2722
• Main Authors: Liu, Pei-Feng, Cheng, Jin-Shiung, Sy, Cheng-Len, Huang, Wei-Chun, Yang, Hsiu-Chen, Gallo, Richard L., Huang, Chun-Ming, Shu, Chih-Wen
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.11.2015; Elsevier Limited
• Subjects: Animals; Antigens, Bacterial - immunology; Autophagy - immunology; Cell Survival - immunology; Cross Infection - microbiology; Cross Infection - prevention & control; Disease Models, Animal; Female; Methicillin-Resistant Staphylococcus aureus - cytology; Methicillin-Resistant Staphylococcus aureus - immunology; Mice; Mice, Inbred ICR; Random Allocation; Sensitivity and Specificity; Staphylococcus aureus; Staphylococcus aureus - pathogenicity; Streptococcus; Virulence Factors
• ISSN: 0022-202X; 1523-1747; 1523-1747
• DOI: 10.1038/jid.2015.254

Methicillin-resistant Staphylococcus aureus (MRSA) has emerged as a major nosocomial pathogen that is widespread in both health-care facilities and in the community at large, as a result of direct host-to-host transmission. Several virulence factors are associated with pathogen transmission to naive hosts. Immunodominant surface antigen B (IsaB) is a virulence factor that helps Staphylococcus aureus to evade the host defense system. However, the mechanism of IsaB on host transmissibility remains unclear. We found that IsaB expression was elevated in transmissible MRSA. Wild-type isaB strains inhibited autophagic flux to promote bacterial survival and elicit inflammation in THP-1 cells and mouse skin. MRSA isolates with increased IsaB expression showed decreased autophagic flux, and the MRSA isolate with the lowest IsaB expression showed increased autophagic flux. In addition, recombinant IsaB rescued the virulence of the isaB deletion strain and increased the group A streptococcus (GAS) virulence in vivo. Together, these results reveal that IsaB diminishes autophagic flux, thereby allowing MRSA to evade host degradation. These findings suggest that IsaB is a suitable target for preventing or treating MRSA infection.