Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry as an Alternative to 16S rRNA Gene Sequencing for Identification of Difficult-To-Identify Bacterial Strains

Conventional methods are sometimes insufficient to identify human bacterial pathogens, and alternative techniques, often molecular, are required. Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) identified with a valid score 45.9% of 410 clinical isolates f...

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Published inJournal of Clinical Microbiology Vol. 49; no. 2; pp. 693 - 696
Main Authors Bizzini, A, Jaton, K, Romo, D, Bille, J, Prod'hom, G, Greub, G
Format Journal Article
LanguageEnglish
Published United States American Society for Microbiology 01.02.2011
SeriesNotes
Subjects
Bacteria
Bacteria - chemistry
Bacteria - classification
Bacteria - isolation & purification
Bacterial Infections - diagnosis
Bacterial Infections - microbiology
Bacteriological Techniques - methods
Bacteriology
DNA, Bacterial - chemistry
DNA, Bacterial - genetics
DNA, Ribosomal - chemistry
DNA, Ribosomal - genetics
Humans
RNA, Ribosomal, 16S - genetics
Sequence Analysis, DNA
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods
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Summary:Conventional methods are sometimes insufficient to identify human bacterial pathogens, and alternative techniques, often molecular, are required. Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) identified with a valid score 45.9% of 410 clinical isolates from 207 different difficult-to-identify species having required 16S rRNA gene sequencing. MALDI-TOF MS might represent an alternative to 16S rRNA gene sequencing.
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G. Prod'hom and G. Greub made equal contributions to this study.
Present address: Clinique de la Source, Avenue Vinet 30, 1004 Lausanne, Switzerland.
ISSN:0095-1137
1098-660X
DOI:10.1128/JCM.01463-10