Genetic diversity of cytochrome P450 2A with different metabolic activities in domestic cats
Knowledge of genetic polymorphisms of cytochrome P450 (CYP), the most important xenobiotic metabolizing enzyme, is very limited in cats. Preliminarily, we investigated genetic polymorphisms in CYP2A13, one of the major CYP isoforms in the liver and lung. Four synonymous and three non-synonymous poly...
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Published in | Journal of Veterinary Medical Science Vol. 81; no. 7; pp. 983 - 985 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
Japan
JAPANESE SOCIETY OF VETERINARY SCIENCE
01.07.2019
Japan Science and Technology Agency The Japanese Society of Veterinary Science |
Subjects | |
Online Access | Get full text |
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Summary: | Knowledge of genetic polymorphisms of cytochrome P450 (CYP), the most important xenobiotic metabolizing enzyme, is very limited in cats. Preliminarily, we investigated genetic polymorphisms in CYP2A13, one of the major CYP isoforms in the liver and lung. Four synonymous and three non-synonymous polymorphic variants were identified in feline CYP2A13 in domestic cats in Japan, without an obvious major type. Metabolic parameters, Km and Vmax, of coumarin hydroxylation of CYP2A13 were shown to range within two times for the identified non-synonymous polymorphic variants by using heterologous coexpression system in Escherichia coli. The results confirmed the polymorphic nature of CYP2A13 as a basis for effective application of medicines and prevention of adverse reactions in treatment of domestic cats. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0916-7250 1347-7439 |
DOI: | 10.1292/jvms.19-0107 |