[11C]carfentanil PET imaging for studying the peripheral opioid system in vivo: effect of photoperiod on mu-opioid receptor availability in brown adipose tissue

• Published in: European journal of nuclear medicine and molecular imaging Vol. 50; no. 2; pp. 266 - 274
• Main Authors: Sun, Lihua, Aarnio, Richard, Herre, Erika Atencio, Kärnä, Salli, Palani, Senthil, Virtanen, Helena, Liljenbäck, Heidi, Virta, Jenni, Honkaniemi, Aake, Oikonen, Vesa, Han, Chunlei, Laurila, Sanna, Bucci, Marco, Helin, Semi, Yatkin, Emrah, Nummenmaa, Lauri, Nuutila, Pirjo, Tang, Jing, Roivainen, Anne
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.01.2023; Springer Nature B.V
• Subjects: Adipose tissue; Adipose tissue (brown); Adipose Tissue, Brown - diagnostic imaging; Adipose Tissue, Brown - metabolism; Analgesics, Opioid - metabolism; Animals; Availability; Body fat; Body mass; Cardiology; Food intake; Imaging; Immunofluorescence; Innervation; Mammals; Medicin och hälsovetenskap; Medicine; Medicine & Public Health; Muscles; Narcotics; Neuroimaging; Nuclear Medicine; Oncology; Opioid receptors (type mu); Original; Original Article; Orthopedics; Photoperiod; Photoperiods; Positron emission; Positron emission tomography; Positron-Emission Tomography - methods; Preclinical Imaging; Radiology; Rats; Receptors; Receptors, Opioid - metabolism; Seasonal variations; Thermogenesis; Brown adipose tissue; Photoperiod; Carfentanil; Mu-opioid receptor; Positron emission tomography
• ISSN: 1619-7070; 1619-7089; 1619-7089
• DOI: 10.1007/s00259-022-05969-5

Purpose Photoperiod determines the metabolic activity of brown adipose tissue (BAT) and affects the food intake and body mass of mammals. Sympathetic innervation of the BAT controls thermogenesis and facilitates physiological adaption to seasonal changes, but the exact mechanism remains elusive. Previous studies have shown that central opioid signaling regulates BAT thermogenesis, and that the expression of the brain mu-opioid receptor (MOR) varies seasonally. Therefore, it is important to know whether MOR expression in BAT shows seasonal variation. Methods We determined the effect of photoperiod on BAT MOR availability using [ 11 C]carfentanil positron emission tomography (PET). Adult rats ( n  = 9) were repeatedly imaged under various photoperiods in order to simulate seasonal changes. Results Long photoperiod was associated with low MOR expression in BAT ( β  =  − 0.04, 95% confidence interval: − 0.07, − 0.01), but not in muscles. We confirmed the expression of MOR in BAT and muscle using immunofluorescence staining. Conclusion Photoperiod affects MOR availability in BAT. Sympathetic innervation of BAT may influence thermogenesis via the peripheral MOR system. The present study supports the utility of [ 11 C]carfentanil PET to study the peripheral MOR system.