Clonal mast cell disorders in patients with systemic reactions to Hymenoptera stings and increased serum tryptase levels

• Published in: Journal of allergy and clinical immunology Vol. 123; no. 3; pp. 680 - 686
• Main Authors: Bonadonna, Patrizia, Perbellini, Omar, Passalacqua, Giovanni, Caruso, Beatrice, Colarossi, Sabrina, Dal Fior, Daniela, Castellani, Luca, Bonetto, Chiara, Frattini, Francesco, Dama, Annarita, Martinelli, Giovanni, Chilosi, Marco, Senna, Gianenrico, Pizzolo, Giovanni, Zanotti, Roberta
• Format: Journal Article
• Language: English
• Published: New York, NY Mosby, Inc 01.03.2009; Elsevier; Elsevier Limited
• Subjects: Adolescent; Adult; Aged; Allergy and Immunology; anaphylaxis; Anaphylaxis - etiology; Animals; Biological and medical sciences; bone marrow; Bone Marrow - pathology; Child; Clone Cells - pathology; Female; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Humans; Hymenoptera; Hymenoptera venom allergy; Immunopathology; Incidence; Insect Bites and Stings - complications; Male; Mast Cells - pathology; mastocytosis; Mastocytosis - enzymology; Mastocytosis - epidemiology; Mastocytosis - pathology; Medical sciences; Middle Aged; Mutation; Point Mutation - genetics; Point Mutation - immunology; Proto-Oncogene Proteins c-kit - genetics; Proto-Oncogene Proteins c-kit - immunology; Proto-Oncogene Proteins c-kit - metabolism; Sarcoidosis. Granulomatous diseases of unproved etiology. Connective tissue diseases. Elastic tissue diseases. Vasculitis; serum tryptase; Skin Tests; Tryptases - blood; Young Adult; HVA; ISM; sBT; mastocytosis; MC; MMAS; anaphylaxis; SM; BM; serum tryptase; Hymenoptera venom allergy; bone marrow; Indolent systemic mastocytosis; Monoclonal mast cell activation syndrome; Systemic mastocytosis; Mast cell; Serum basal tryptase; Human; Allergy; Immunopathology; Mastocytosis; Serine endopeptidases; Enzyme; Insecta; Sting; Peptidases; Anaphylaxis; Immunology; Arthropoda; Venom; Tryptase; Bone marrow; Hydrolases; Serum; Invertebrata; Hymenoptera
• ISSN: 0091-6749; 1097-6825; 1097-6825
• DOI: 10.1016/j.jaci.2008.11.018

Anaphylaxis after Hymenoptera stings has been reported in subjects with mastocytosis, but few data exist regarding disease prevalence in populations allergic to these insects. The incidence of clonal mast cell (MC) disorders in subjects with both systemic reactions to Hymenoptera stings and increased serum baseline tryptase (sBT) levels was assessed by using bone marrow (BM) aspirates and biopsy specimens. Subjects with a history of a systemic reaction caused by a Hymenoptera sting underwent the standard diagnostic work-up for Hymenoptera allergy, and sBT levels were measured. Subjects with an increased sBT level had BM evaluation that included histology/cytology, flow cytometry, and detection of KIT mutations. Forty-four (11.6%) of 379 subjects with systemic reactions had increased sBT levels (>11.4 ng/mL), and 31 (70.5%) of these had a history of anaphylaxis. Thirty-four subjects with increased sBT levels underwent a BM analysis. Histology detected diagnostic or subdiagnostic MC infiltrates in 22 (65%) of 34 patients. Abnormal MCs were identified by means of flow cytometry and cytology in 26 (78.8%) of 33 and 20 (58.8%) of 34 subjects, respectively. A KIT mutation was detected in 17 (54.8%) of 31 subjects. The diagnosis was indolent systemic mastocytosis in 21 (61.7%) of 34 subjects and monoclonal MC activation syndrome in 9 (26.5%) of 34 subjects. All subjects with anaphylaxis had one of those 2 disorders. The concomitant presence of systemic reactions (especially anaphylaxis) after Hymenoptera stings and increased sBT levels strongly suggests that a BM examination is indicated for the diagnosis of clonal MC disease.