Development of a one-run real-time PCR detection system for pathogens associated with poultry infectious diseases

• Published in: Journal of Veterinary Medical Science Vol. 85; no. 4; pp. 407 - 411
• Main Authors: SHIBANUMA, Takuya, NUNOMURA, Yuka, OBA, Mami, KAWAHARA, Fumiya, MIZUTANI, Tetsuya, TAKEMAE, Hitoshi
• Format: Journal Article
• Language: English
• Published: Japan JAPANESE SOCIETY OF VETERINARY SCIENCE 2023; Japan Science and Technology Agency; The Japanese Society of Veterinary Science
• Subjects: Animals; Avian Pathology; chicken; Chickens - microbiology; Communicable Diseases - veterinary; DNA viruses; Female; Genomes; infectious disease; Infectious diseases; pathogen detection system; Pathogens; Polymerase chain reaction; Poultry; Poultry Diseases; real-time PCR; Real-Time Polymerase Chain Reaction - veterinary; RNA Viruses; chicken; poultry; real-time PCR; infectious disease; pathogen detection system

Infectious diseases are an important issue in the poultry industry, requiring early diagnosis and countermeasures. To address this, we present a system based on TaqMan real-time PCR to detect pathogen genome in specimens collected from chickens. We designed 12 primer–probe sets for pathogens causing...