Development of a one-run real-time PCR detection system for pathogens associated with poultry infectious diseases

• Published in: Journal of Veterinary Medical Science Vol. 85; no. 4; pp. 407 - 411
• Main Authors: SHIBANUMA, Takuya, NUNOMURA, Yuka, OBA, Mami, KAWAHARA, Fumiya, MIZUTANI, Tetsuya, TAKEMAE, Hitoshi
• Format: Journal Article
• Language: English
• Published: Japan JAPANESE SOCIETY OF VETERINARY SCIENCE 2023; Japan Science and Technology Agency; The Japanese Society of Veterinary Science
• Subjects: Animals; Avian Pathology; chicken; Chickens - microbiology; Communicable Diseases - veterinary; DNA viruses; Female; Genomes; infectious disease; Infectious diseases; pathogen detection system; Pathogens; Polymerase chain reaction; Poultry; Poultry Diseases; real-time PCR; Real-Time Polymerase Chain Reaction - veterinary; RNA Viruses; chicken; poultry; real-time PCR; infectious disease; pathogen detection system
• ISSN: 0916-7250; 1347-7439; 1347-7439
• DOI: 10.1292/jvms.22-0482

Infectious diseases are an important issue in the poultry industry, requiring early diagnosis and countermeasures. To address this, we present a system based on TaqMan real-time PCR to detect pathogen genome in specimens collected from chickens. We designed 12 primer–probe sets for pathogens causing respiratory or systemic symptoms. In field samples, we detected three viruses, including DNA and RNA viruses, and three bacteria. The chicken anemia virus and Avibacterium paragallinarum were detected only in young and laying hens, respectively. Bacteria were detected only in throat swabs, and gallid alphaherpesvirus 2 was detected in different specimens at each developmental stage. Our novel TaqMan real-time PCR system effectively detects pathogen’s gene in chickens, while taking age into account.