Seroprevalence of human brucellosis and molecular characteristics of Brucella strains in Inner Mongolia Autonomous region of China, from 2012 to 2016

• Published in: Emerging microbes & infections Vol. 9; no. 1; pp. 263 - 274
• Main Authors: Liu, Zhi-guo, Wang, Miao, Ta, Na, Fang, Meng-gang, Mi, Jing-chuan, Yu, Rui-ping, Luo, Yao, Cao, Xiaoan, Li, Zhen-jun
• Format: Journal Article
• Language: English
• Published: United States Taylor & Francis 01.01.2020; Taylor & Francis Ltd; Taylor & Francis Group
• Subjects: Alleles; Brucella - genetics; Brucella melitensis; Brucellosis; Brucellosis - epidemiology; China - epidemiology; Genotype; Humans; Inner Mongolia; MLVA; molecular epidemiology; Phylogeny; Seroepidemiologic Studies; Serology; Time Factors; China; Inner Mongolia China; molecular epidemiology; Serology; Inner Mongolia; Brucella melitensis; MLVA
• ISSN: 2222-1751; 2222-1751
• DOI: 10.1080/22221751.2020.1720528

In the present study, a total of 1102304 serum samples were collected to detected human brucellosis between the years 2012 and 2016 in Inner Mongolia. Overall, an average of 3.79% anti-Brucella positive in Inner Mongolia was presented but the range of positive rates were among 0.90 to 7.07% in 12 regions. Seroprevalence of human brucellosis increased gradually from 2012 to 2016. However, the incidence rate of human brucellosis showed a declining trend. One hundred and seven Brucella strains were isolated and identified as B. melitensis species, and B. melitensis biovar 3 was the predominant biovar. MLVA-11 genotypes 116 was predominant and had crucial epidemiology to the human population. All 107 strains tested were sorted into 75 MLVA-16 genotypes, with 54 single genotypes representing unique isolates. This result revealed that these Brucellosis cases had epidemiologically unrelated and sporadic characteristics. The remaining 21 shared genotypes among two to four strains, confirming the occurrence of cross-infection and multiple outbreaks. Extensive genotype-events were observed between strains from this study and Kazakhstan, Mongolia, and Turkey, these countries were key members of the grassland silk road. Long-time trade in small ruminants (sheep) in these countries has possibly promoted the spread of Brucella spp. in these regions.