Comparison of antigenicity between recently isolated bovine rotaviruses A and vaccine strains by cross-neutralizing antibody tests and their significance for immunization

• Published in: Journal of Veterinary Medical Science Vol. 85; no. 3; pp. 318 - 325
• Main Authors: HASAN, Md. Amirul, KABIR, Md. Humayun, MIYAOKA, Yu, YAMAGUCHI, Makiko, SHOHAM, Dany, TAKEHARA, Kazuaki
• Format: Journal Article
• Language: English
• Published: Japan JAPANESE SOCIETY OF VETERINARY SCIENCE 2023; Japan Science and Technology Agency; The Japanese Society of Veterinary Science
• Subjects: Animals; Antibodies; Antibodies, Viral; Antigenicity; Antisera; bovine rotavirus A; Broadly Neutralizing Antibodies; Cattle; Cattle Diseases; Colostrum; Diarrhea; Female; Immunization; Immunization - veterinary; Neutralization Tests - veterinary; Rotavirus; Rotavirus Infections - veterinary; serotype; Serotypes; vaccine; Vaccines; Virology; virus isolation; virus neutralization test; bovine rotavirus A; virus isolation; vaccine; virus neutralization test; serotype
• ISSN: 0916-7250; 1347-7439
• DOI: 10.1292/jvms.22-0510

Three bovine rotaviruses A (RVAs) isolated from a cattle farm in Japan were serotyped by serum neutralization assay, as compared with the RVA strains contained in a vaccine used on the same farm. Antisera were prepared against the three isolates and the vaccine strains of bovine RVA. The results of cross-neutralization tests revealed that the RVA isolates from this farm differed somewhat in serotype. Collected plasma from calves for 6 weeks after colostrum ingestion showed that maternal antibodies acquired against all isolates gradually decreased, but antibodies toward one isolate increased by 6 weeks after the mentioned decreasing. These results suggest that rotavirus vaccines administered to cows should include all serotypes commonly found in calves with diarrhea.