Levels and complexity of IgA antibody against oral bacteria in samples of human colostrum

• Published in: Immunobiology (1979) Vol. 220; no. 1; pp. 142 - 146
• Main Authors: Petrechen, L.N, Zago, F.H, Sesso, M.L.T, Bertoldo, B.B, Silva, C.B, Azevedo, K.P, de Lima Pereira, S.A, Geraldo-Martins, V.R, Ferriani, V.P.L, Nogueira, R.D
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier GmbH 01.01.2015
• Subjects: Adult; Advanced Basic Science; Allergy and Immunology; Antigens, Bacterial - immunology; Bacteria - immunology; Bacteria - isolation & purification; Colostrum; Colostrum - immunology; Female; Humans; IgA; Immunoglobulin A, Secretory - immunology; Immunoglobulin G - immunology; Immunoglobulin M - immunology; Milk, Human - immunology; Mucosal immunity; Streptococcus gordonii; Streptococcus mitis; Streptococcus mutans; Streptococcus sanguinis; Virulence Factors - immunology; IgA; Mucosal immunity; Colostrum; Streptococcus mutans
• ISSN: 0171-2985; 1878-3279
• DOI: 10.1016/j.imbio.2014.08.009

Abstract Streptococcus mutans (SM) have three main virulence antigens: glucan binding protein B (gbpB), glucosyltransferase (Gtf) and antigens I/II (Ag I/II) envolved in the capacity of those bacteria to adhere and accumulate in the dental biofilm. Also, the glycosyltransferases 153 kDa of Streptococcus gordonii (SGO) and 170 kDa of Streptococcus sanguinis (SSA) were important antigens associated with the accumulation of those bacterias. Streptococcus mitis (SMI) present IgA1 protease of 202 kDa. We investigated the specificity and levels IgA against those antigens of virulence in samples of human colostrum. This study involved 77 samples of colostrum that were analyzed for levels of immunoglobulian A, M and G by Elisa. The specificity of IgA against extracts of SM and initials colonizators (SSA, SMI, SGO) were analyzed by the Western blot. The mean concentration of IgA was 2850.2 (±2567.2) mg/100 mL followed by IgM and IgG (respectively 321.8 ± 90.3 and 88.3 ± 51.5), statistically different ( p < 0.05). Results showed that the majority of samples had detectable levels of IgA antibodies to extracts of bacteria antigens and theirs virulence antigens. To SM, the GbpB was significantly lower detected than others antigens of SM ( p < 0.05). High complexities of response to Ags were identified in the samples. There were no significant differences in the mean number of IgA-reactive Ags between the antigens ( p > 0.4). So, the breast milk from first hours after birth presented significant levels of IgA specific against important virulence of antigens those oral streptococci, which can disrupt the installation and accumulation process of these microorganisms in the oral cavity.