Application of gel permeation HPLC for lipoprotein profiling in dogs

• Published in: Journal of Veterinary Medical Science Vol. 72; no. 6; pp. 813 - 817
• Main Authors: Mizutani, H., Nippon Veterinary and Life Science Univ., Musashino, Tokyo (Japan), Sako, T, Arai, N, Kuriyama, K, Yoshimura, I, Mori, A, Iwase, K, Hirose, H
• Format: Journal Article
• Language: English
• Published: Japan JAPANESE SOCIETY OF VETERINARY SCIENCE 01.06.2010; Japan Science and Technology Agency
• Subjects: Animals; canine; CHIEN; CHOLESTEROL; Chromatography, Gel - methods; Chromatography, High Pressure Liquid - methods; COLESTEROL; DIABETE; DIABETES; DOGS; Dogs - blood; HPLC; http://www.fao.org/aos/agrovoc#c_10361; http://www.fao.org/aos/agrovoc#c_14992; http://www.fao.org/aos/agrovoc#c_1584; http://www.fao.org/aos/agrovoc#c_2352; http://www.fao.org/aos/agrovoc#c_24469; http://www.fao.org/aos/agrovoc#c_4367; lipoprotein profile; LIPOPROTEINAS; LIPOPROTEINE; LIPOPROTEINS; Lipoproteins - blood; Lipoproteins, HDL - blood; Lipoproteins, HDL - isolation & purification; Lipoproteins, LDL - blood; Lipoproteins, LDL - isolation & purification; Lipoproteins, VLDL - blood; Lipoproteins, VLDL - isolation & purification; PERRO; Reference Values; TRIGLICERIDOS; TRIGLYCERIDE; TRIGLYCERIDES; Triglycerides - blood; Triglycerides - isolation & purification; Ultracentrifugation - methods
• ISSN: 0916-7250; 1347-7439
• DOI: 10.1292/jvms.09-0500

A high performance liquid chromatography system with a gel permeation column (GP-HPLC) and an on-line dual enzymatic system was applied to lipoprotein analysis in dogs. A high density lipoprotein (HDL) fraction obtained by conventional ultracentrifugation gave a single peak at around 28- 29 min. Similarly, a low density lipoprotein (LDL) fraction gave single peak at around 24-25 min. The lipoprotein profiles of healthy dogs were contained large HDL peaks and small LDL peaks, and VLDL and CM were only marginally detected. In diabetic dogs, concentrations of VLDL-triglyceride and VLDL-total-cholesterol were elevated significantly. The lipoprotein profile analysis by GP-HPLC method would be useful in explication of abnormality of lipid metabolism in dogs.